The objective of the current work was to investigate the potential of halophilic bacterial isolates for efficient utilization of crude glycerol from algal biodiesel waste into polyhydroxyalkanoates (PHAs) a green plastic. Screening of the isolates was directly done in algal biodiesel waste residue containing solid agar plates supplemented with Nile red. Crude glycerol is a biodiesel waste whose bioconversion into value-added products provides an alternative for efficient management with dual benefit. For the scale-up studies of PHAs, Halomonas spp. especially H. daqingensis was observed as a potential candidate growing well in 3% Algal biodiesel waste residue (ABWR), 5% NaCl supplementation at 35°C within 48 h of incubation. Maximum Cell dry weight (CDW) of 0.362 ± 0.001 g and 0.236 ± 0.003 g PHA was obtained with H. daqingensis when grown in the fermentor with 0.5 vvm air flow rate and 200 rpm containing 3% ABWR supplemented with 5% NaCl at 35°C incubation temperature for 48 h. ABWR can serve as a sole substrate for PHA production at an industrial scale serving two approaches: getting rid of the biodiesel industrial waste containing high amount of glycerol besides using waste replacing commercial substrate thereby reducing the cost of the product.

当前工作的目的是研究嗜盐细菌分离物将藻类生物柴油废物中的粗甘油有效利用为绿色塑料聚羟基链烷酸酯 (PHA) 的潜力。分离物的筛选直接在含有补充有尼罗红的固体琼脂平板的藻类生物柴油废渣中进行。粗甘油是一种生物柴油废物，其生物转化为增值产品为具有双重效益的高效管理提供了替代方案。对于 PHA 的放大研究，盐单胞菌属 尤其 大庆红豆杉在 3% 藻类生物柴油废渣 (ABWR)、5% NaCl 补充剂中，在 35°C 下孵育 48 小时，观察到作为潜在候选者生长良好。最大细胞干重 (CDW) 为 0.362 ± 0.001 g 和 0.236 ± 0.003 g PHA大庆红豆杉当在 0.5 vvm 空气流速和 200 rpm 的发酵罐中生长时，含有 3% ABWR 并辅以 5% NaCl，在 35°C 孵育温度下培养 48 小时。ABWR 可以作为工业规模 PHA 生产的唯一底物，提供两种方法：除使用废物代替商业底物外，去除含有大量甘油的生物柴油工业废物，从而降低产品成本。