In the mammalian genome, DNA methylation is an epigenetic mechanism involving the transfer of a methyl group onto the C5 position of the cytosine to form 5-methylcytosine. DNA methylation regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factors (TFs) to DNA. As there are still many questions concerning the role of methylation in creating personality, we concentrated on searching for such associations. The research group was 100 sports male subjects (mean age = 22.88, SD = 6.35), whereas the control group included 239 healthy male volunteers matched for age (mean age = 21.69, SD = 3.39), both of European origin. The methods used in our research were as follows: DNA isolation, methylation-specific PCR, sequencing chromatophores, all conducted according to the manufacturer’s procedure. To evaluate personality traits, the NEO Five-Factor Personality Inventory (NEO-FFI) and STAI Inventory were used. We observed the existence of a statistically significant correlation for all the aspects of personality covered and CpG islands’ methylation. Nonetheless, we think that the tested group and the number of tested promotor islands in the DAT1 gene are still too small to make explicit conclusions, so it needs further profound analysis.

中文翻译：

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DAT1 启动子甲基化对运动表现的影响

在哺乳动物基因组中，DNA 甲基化是一种表观遗传机制，涉及将甲基转移到胞嘧啶的 C5 位置以形成 5-甲基胞嘧啶。DNA 甲基化通过招募参与基因抑制的蛋白质或通过抑制转录因子 (TF) 与 DNA 的结合来调节基因表达。由于关于甲基化在创造人格中的作用仍有许多问题，我们专注于寻找这种关联。研究组是 100 名运动男性受试者（平均年龄 = 22.88，SD = 6.35），而对照组包括 239 名年龄相匹配的健康男性志愿者（平均年龄 = 21.69，SD = 3.39），均为欧洲血统。我们研究中使用的方法如下：DNA分离、甲基化特异性PCR、染色质测序、全部按照制造商的程序进行。为了评估人格特质，使用了NEO五因素人格量表（NEO-FFI）和STAI量表。我们观察到人格所涵盖的所有方面和 CpG 岛的甲基化存在统计学上显着的相关性。尽管如此，我们认为测试组和测试启动子岛的数量DAT1基因仍然太小，无法做出明确的结论，因此需要进一步深入分析。