Okra (Abelmoschus esculentus L. Moench) is considered as a treasure house of nutrients and it is one of the major vegetables widely spread all over tropical, subtropical and warm temperate regions of the world. Yellow vein mosaic virus (YVMV) and enation leaf curl virus are the most destructive diseases of okra as they affect both crop growth and yield. Due to the frequent breakdown of resistance and lack of a stable source of resistance in the cultivated species, interspecific hybridization is considered as a reliable approach for durable resistance. Cultivated species from The United States Department of Agriculture and wild accessions from The National Bureau of Plant Genetic Resources were screened at YVMV hotspot (Guntur, Andhra Pradesh) to identify the potential donors for disease resistance. Accessions IC141032 and IC141012 were found to be free from both viruses and categorized as resistant lines. Interspecific hybridization between A. tetraphyllus and A. esculentus revealed a high crossability index of around 80% when A. esculentus was utilized as a female parent. The bottleneck of hybrid sterility was partially overcome by the colchicine treatment of interspecific F1 hybrids. Good seed set was observed when raw colchiploids were backcrossed to the recurrent parent.

中文翻译：

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种间杂交作为对秋葵病毒的抗性转移的一种方式：障碍和前进的方向

秋葵 （南芥L. Moench）被认为是营养的宝库，是主要蔬菜之一，广泛分布于世界热带、亚热带和暖温带地区。黄脉花叶病毒（YVMV）和卷叶病毒是秋葵最具破坏性的病害，因为它们影响作物生长和产量。由于栽培物种中抗性的频繁破坏和缺乏稳定的抗性来源，种间杂交被认为是持久抗性的可靠方法。在 YVMV 热点（安得拉邦贡图尔）筛选来自美国农业部的栽培物种和来自国家植物遗传资源局的野生种质，以确定潜在的抗病供体。发现收集品 IC141032 和 IC141012 不含这两种病毒并被归类为抗性品系。种间杂交四叶草和A. esculentus显示出约 80% 的高交叉性指数时A. esculentus被用作女性父母。通过秋水仙碱处理种间 F1 杂种，部分克服了杂种不育的瓶颈。当原始的colchiploids与轮回亲本回交时观察到良好的结实。