Biostimulants are receiving increasing attention for their beneficial effects on crops, driving interest in identifying new plant extracts that could exert such stimulatory effects. This work aimed to evaluate the potential of an aqueous extract obtained from duckweed (Lemna minor L.), a freshwater species, to act as a biostimulant in maize. For this purpose, duckweed plants were collected from a natural basin and then transferred, stabilized, and grown under controlled conditions. The duckweed extract was first characterized through untargeted profiling, which revealed an abundance of bioactive phytochemicals. A relatively high amount of low-molecular-weight secondary metabolites such as phenolics (6714.99 mg kg⁻¹) and glucosinolates (4563.74 mg kg⁻¹) were present in the plant extract. Maize seeds were primed with different concentrations of this extract (0.01%, 0.05%, 0.50%, and 1.00%, dry weight/water volume), and some physiological and biochemical traits of the crop were recorded. The duckweed extract improved maize germination, biomass, leaf area, pigment content, and vigor index. The most effective treatment was the 0.50% concentration, which improved the majority of the measured growth traits. The extract at concentrations of 0.05%, 0.50%, and 1.00% stimulated the assimilation of nitrogen (N), phosphorous (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), iron (Fe), and copper (Cu). In summary, this study revealed that duckweed is a promising species that can be cultured and grown under controlled conditions for obtaining extracts with biostimulant properties.

生物刺激剂因其对作物的有益作用而受到越来越多的关注，这推动了人们对确定可以发挥这种刺激作用的新植物提取物的兴趣。这项工作旨在评估从浮萍（Lemna minor L.）（一种淡水物种）中提取的水提取物作为玉米生物刺激剂的潜力。为此，从天然盆中收集浮萍植物，然后在受控条件下转移、稳定和生长。浮萍提取物首先通过非靶向分析进行表征，揭示了丰富的生物活性植物化学物质。相对大量的低分子量次级代谢物，如酚类物质 (6714.99 mg kg ^-1 ) 和硫代葡萄糖苷 (4563.74 mg kg ^-1) 存在于植物提取物中。玉米种子用不同浓度的这种提取物（0.01%、0.05%、0.50% 和 1.00%，干重/水体积）引发，并记录了作物的一些生理和生化性状。浮萍提取物改善了玉米发芽、生物量、叶面积、色素含量和活力指数。最有效的处理是 0.50% 的浓度，它改善了大部分测量的生长性状。浓度为 0.05%、0.50% 和 1.00% 的提取物刺激了氮 (N)、磷 (P)、钾 (K)、钙 (Ca)、镁 (Mg)、钠 (Na)、铁 ( Fe) 和铜 (Cu)。总之，这项研究表明浮萍是一种很有前途的物种，可以在受控条件下培养和生长，以获得具有生物刺激特性的提取物。