Journal of Natural Medicines ( IF 2.5 ) Pub Date : 2021-09-16 , DOI: 10.1007/s11418-021-01562-6 Ulises G Castillo 1 , Ayato Komatsu 2 , Morena L Martínez 1 , Jenny Menjívar 3 , Marvin J Núñez 1 , Yoshinori Uekusa 2 , Yuji Narukawa 2 , Fumiyuki Kiuchi 2 , Junko Nakajima-Shimada 4
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Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and in Central America, it is considered one of the four most infectious diseases. This study aimed to screen the anti-trypanosomal activity of plant species from Salvadoran flora. Plants were selected through literature search for plants ethnobotanically used for antiparasitic and Chagas disease symptomatology, and reported in Museo de Historia Natural de El Salvador (MUHNES) database. T. cruzi was incubated for 72 h with 2 different concentrations of methanolic extracts of 38 species, among which four species, Piper jacquemontianum, Piper lacunosum, Trichilia havanensis, and Peperomia pseudopereskiifolia, showed the activity (≤ 52.0% viability) at 100 µg/mL. Separation of the methanolic extract of aerial parts from Piper jacquemontianum afforded a new flavanone (4) and four known compounds, 2,2-dimethyl-6-carboxymethoxychroman-4-one (1), 2,2-dimethyl-6-carboxychroman-4-one (2), cardamomin (3), and pinocembrin (5), among which cardamomin exhibited the highest anti-trypanosomal activity (IC50 = 66 µM). Detailed analyses of the spectral data revealed that the new compound 4, named as jaqueflavanone A, was a derivative of pinocembrin having a prenylated benzoate moiety at the 8-position of the A ring.
Graphic abstract
中文翻译：
萨尔瓦多菌群的抗锥虫筛选
南美锥虫病是由原生动物寄生虫克氏锥虫引起的，在中美洲，它被认为是四大传染性疾病之一。本研究旨在筛选来自萨尔瓦多植物群的植物物种的抗锥虫活性。通过文献搜索植物学用于抗寄生虫和南美锥虫病症状学的植物，并在萨尔瓦多自然历史博物馆 (MUHNES) 数据库中报告植物。T. cruzi用 38 个物种的 2 个不同浓度的甲醇提取物孵育 72 h，其中 4 个物种，Piper jacquemontianum，Piper lacunosum，Trichilia havanensis和Peperomia pseudopereskiifolia, 在 100 µg/mL 时显示活动（≤ 52.0% 的生存能力）。从Piper jacquemontianum地上部分的甲醇提取物中分离得到一种新的黄烷酮 ( 4 ) 和四种已知化合物，2,2-二甲基-6-羧基甲氧基色满-4-酮 ( 1 )、2,2-二甲基-6-羧基色满- 4-one ( 2 )、cardamomin ( 3 ) 和 pinocembrin ( 5 )，其中 cardamomin 表现出最高的抗锥虫活性 (IC 50 = 66 µM)。对光谱数据的详细分析表明，新化合物4命名为 jaqueflavanone A，是松树素的衍生物，在 A 环的 8 位具有异戊二烯基苯甲酸酯基团。
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