Abstract

The majority of the naturally occurring antioxidants are obtained from plant sources. The antioxidant activity is mostly exhibited by polyphenols present in the plant cells. Azadirachta Indica (Neem) leaves are renowned for their medicinal applications due to their anti-inflammatory, antimalarial, antifungal, antibacterial, antiviral, antioxidant, and anticarcinogenic properties. This work aims to optimize the extraction of Azadirachta Indica (Neem) leaf antioxidants using three-phase partitioning (TPP). The optimized conditions are operating time 15 minutes, slurry ratio 1:30 (g/mL), salt concentration 30% (w/v), aqueous to solvent ratio 1:1.5 (v/v), and stirring speed 400 rpm that infer 74.66% extraction yield. Additionally, ultrasonic pretreatment was also employed to increase the extraction yield up to 86.61%. Sonication pretreatment for 4 min operated at 30 W power, and 75% duty cycle was observed to offer maximum antioxidant extraction about 3.3 mg/g.

摘要

大多数天然存在的抗氧化剂来自植物来源。植物细胞中存在的多酚主要表现出抗氧化活性。Azadirachta Indica (Neem) 叶子因其抗炎、抗疟、抗真菌、抗菌、抗病毒、抗氧化和抗癌特性而闻名。这项工作旨在优化使用三相分配 (TPP)提取印楝(Neem) 叶抗氧化剂。优化条件为运行时间 15 分钟，浆料比 1:30 (g/mL)，盐浓度 30% ( w/v )，水溶剂比 1:1.5 ( v/v )), 搅拌速度为 400 rpm, 推断提取率为 74.66%。此外，还采用超声预处理将提取率提高到 86.61%。在 30 W 功率下运行 4 分钟的超声预处理，观察到 75% 的占空比可提供约 3.3 mg/g 的最大抗氧化剂提取。