Abstract

A new unsaturated fatty acid trewioidesine A (1), together with seven known compounds (2 − 8) were isolated from the rhizomes of Alchornea trewioides (Benth.) Muell. Arg. Their structures were established on the basis of extensive spectroscopic data interpretation (1 D and 2 D NMR, and HRESIMS). The absolute configuration of 1 was determined by electronic circular dichroism (ECD) calculations, confirming as trewioidesine A. The functionality of isolated compounds was tested in cultured PC12 cells, a cell line from rat pheochromocytoma. Trewioidesine A was the one showing robust activity in inducing neuronal differentiation: the induction was synergized when co-applied with nerve growth factor (NGF). In addition, a neurofilament 200 (NF200) promoter-luciferase (pNF200-Luc) reporter was used to evaluate the differentiating ability in the transfected PC12 cells for the isolated compounds. Trewioidesine A exhibited a strong NF200 promoter activation, and application of trewioidesine A with low dose of NGF significantly induced the promoter activity over 50%.

摘要

从Alchornea trewioides (Benth.) Muell的根茎中分离出一种新的不饱和脂肪酸 trewioidesine A ( 1 ) 以及七种已知化合物 ( 2  -  8 )。精氨酸。它们的结构是在广泛的光谱数据解释（一维和二维核磁共振以及 HRESIMS）的基础上建立的。1的绝对配置通过电子圆二色谱 (ECD) 计算确定，确认为 trewioidesine A。在培养的 PC12 细胞（一种来自大鼠嗜铬细胞瘤的细胞系）中测试了分离化合物的功能。Trewioidesine A 是在诱导神经元分化方面表现出强大活性的一种：当与神经生长因子 (NGF) 共同应用时，诱导作用得到协同。此外，使用神经丝 200 (NF200) 启动子-荧光素酶 (pNF200-Luc) 报告基因来评估转染的 PC12 细胞中分离化合物的分化能力。Trewioidesine A 表现出强烈的 NF200 启动子活化，并且应用低剂量 NGF 的 trewioidesine A 显着诱导启动子活性超过 50%。