Globally, pre‑eclampsia (PE) is a gestational disorder that causes increased morbidity of the fetus and mortality induced by pregnancy. Despite various studies, the understanding of the causes or mechanism of the development of PE remains elusive. Thus, the present study aimed to investigate the role of circular (circ)RNA hsa_circ_0026552 (hsa_circ_0026552) in the development of PE and its mechanism of regulation. hsa_circ_0026552 differential expression in PE tissue data and clinical samples were analyzed and it was observed that hsa_circ_0026552 is highly upregulated in PE samples. Furthermore, miR‑331‑3p was detected as an hsa_circ_0026552 target miRNA and TGF‑βR1 gene as a target of miR‑331‑3p. These results were confirmed using various assays, including dual‑luciferase reporter, reverse transcription‑quantitative PCR and RNA pull‑down assay. It was observed that miR‑331‑3p expression was negatively correlated to hsa_circ_0026552 relative expression, while TGF‑βR1 expression was positively correlated to hsa_circ_0026552 expression evaluated by Pearson's correlation test. The functional experiments, including Cell Counting Kit‑8, colony formation and Transwell assay, showed that silencing hsa_circ_0026552 could significantly strengthen the proliferation, migration and invasion of the trophoblastic HTR‑8/SVneo cells, but the subsequent overexpression of hsa_circ_0026552 reversed this. Mechanistically, it was concluded that hsa_circ_0026552 acts as a miR‑331‑3p sponge to upregulate TGF‑βR1 expression in trophoblasts and is involved significantly in PE development and progression in pregnant women. The circRNA hsa_circ_0026552 could be a novel therapeutic target and prognostic biomarker for PE.

中文翻译：

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环状 RNA hsa_circ_0026552 在先兆子痫中通过 miR-331-3p/TGF-βR1 轴抑制滋养层细胞的增殖、迁移和侵袭。

在全球范围内，先兆子痫 (PE) 是一种妊娠疾病，可导致胎儿发病率和妊娠导致的死亡率增加。尽管进行了各种研究，但对 PE 发展的原因或机制的理解仍然难以捉摸。因此，本研究旨在探讨环状（circ）RNA hsa_circ_0026552（hsa_circ_0026552）在PE发展中的作用及其调控机制。分析了PE组织数据和临床样本中hsa_circ_0026552的差异表达，观察到hsa_circ_0026552在PE样本中高度上调。此外，miR-331-3p 被检测为 hsa_circ_0026552 靶 miRNA，TGF-βR1 基因被检测为 miR-331-3p 的靶标。这些结果通过各种检测得到证实，包括双荧光素酶报告基因、逆转录定量 PCR 和 RNA 下拉检测。通过 Pearson 相关性检验，观察到 miR-331-3p 的表达与 hsa_circ_0026552 的相对表达呈负相关，而 TGF-βR1 的表达与 hsa_circ_0026552 的表达呈正相关。包括细胞计数试剂盒-8、集落形成和Transwell检测在内的功能实验表明，沉默hsa_circ_0026552可以显着增强滋养层HTR-8/SVneo细胞的增殖、迁移和侵袭，但随后的hsa_circ_0026552过表达逆转了这一点。机制上，得出的结论是，hsa_circ_0026552 作为 miR-331-3p 海绵上调滋养细胞中 TGF-βR1 的表达，并显着参与孕妇 PE 的发生和进展。