Caffeine, a xenobiotic compound, is continuously released into the environment. Fifteen psychrotolerant bacterial strains, isolated from the Indian Himalayan region, were screened for their caffeine degradation capacity. The medium for the growth of bacteria was optimized using Box-Behnken method. Among these bacteria, Pseudomonassp. (GBPI_Hb5), showing the best response, was further used for caffeine degradation in batch mode. The culture medium, having caffeine as a sole source of carbon, was used for analyzing the effect of pH, agitation speed, temperature, inoculum volume, and caffeine concentration on bacterial growth and its caffeine degradation potential. The bacterium GBPI_Hb5 showed approx. 93% caffeine degradation up to 96 h under controlled conditions. The compounds produced during the degradation of caffeine were also studied. The study is likely to have implications in the bioremediation of caffeine from polluted environments.

咖啡因是一种异生化合物，会不断释放到环境中。筛选了从印度喜马拉雅地区分离的 15 种耐冷细菌菌株的咖啡因降解能力。使用Box-Behnken方法优化用于细菌生长的培养基。在这些细菌中，假单胞菌sp. (GBPI_Hb5) 显示出最佳响应，进一步用于批量模式下的咖啡因降解。将咖啡因作为唯一碳源的培养基用于分析 pH 值、搅拌速度、温度、接种量和咖啡因浓度对细菌生长及其咖啡因降解潜力的影响。细菌 GBPI_Hb5 显示约。在受控条件下，93% 的咖啡因降解长达 96 小时。还研究了咖啡因降解过程中产生的化合物。该研究可能对污染环境中咖啡因的生物修复产生影响。