Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patients with ISR remains unclear. This study aims to profile the BCR repertoire in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire profiles using DNA-seq. Our results showed that the diversity of amino acid sequences in ISR DM patients was higher than that in ISR −DM patients. The frequencies of 21 V/J paired genes differed between ISR DM and −ISR DM patients, while frequencies of 5 V/J paired genes differed between ISR DM and ISR −DM. The −ISR −DM group presented the highest clonotype overlap rate, while ISR DM patients presented the lowest overlap rate. Our study presented the BCR repertoires in patients with ISR/T2DM. The data suggested different BCR signatures between patients with ISR and T2DM. Further analysis of BCR profiles would enhance understanding of ISR.

中文翻译：

---

冠状动脉支架内再狭窄合并 2 型糖尿病患者 B 细胞受体库的克隆多样性

2 型糖尿病 (T2DM) 被认为是冠状动脉疾病 (CAD) 患者冠状动脉支架内再狭窄 (ISR) 的危险因素。有证据表明，B 细胞在动脉粥样硬化病变的进展中发挥功能性作用。然而，ISR 患者的 B 细胞受体 (BCR) 库仍不清楚。本研究旨在分析冠状动脉 ISR/T2DM 患者的 BCR 曲目。共招募了 21 名伴有或不伴有 ISR/T2DM 的 CAD 患者。使用 DNA-seq 分离 PBMC 并检查 BCR 库谱。我们的结果表明，ISR DM 患者的氨基酸序列多样性高于 ISR -DM 患者。ISR DM 和 -ISR DM 患者之间 21 个 V/J 配对基因的频率不同，而 ISR DM 和 ISR -DM 患者之间 5 个 V/J 配对基因的频率不同。-ISR -DM 组表现出最高的克隆型重叠率，而 ISR DM 患者表现出最低的重叠率。我们的研究展示了 ISR/T2DM 患者的 BCR 库。数据表明 ISR 和 T2DM 患者之间存在不同的 BCR 特征。对 BCR 配置文件的进一步分析将增强对 ISR 的理解。