A novel hollow polypyrrole coated by mesoporous silica nanoparticles graft poly(2-(4-formylbenzoyloxy)ethyl methacrylate-co-2-(dimethylamino)ethyl methacrylate)-block-poly(triethylene glycol methyl ether methacrylate) multifunctional nanocomposite (HPPy@MSN-SS-NH-g-P(FBEMA-co-DMAEMA)-b-PTEGMA) was designed and prepared via disulfide bond linkages. This material as a gatekeeper integrated multifunctionality such as pore capping, and pH and potothermal multistimuli response abilities, and possessed good photothermal stability, near-infrared (NIR) photothermal conversion capacity and dispersion stability in aqueous solution. It could entrap anticancer doxorubicin (DOX) by pore channels of HPPy@MSN, the core of amphiphilic copolymer micelles and Schiff base linkages, offering a load capacity and load efficiency of up to about 41.5 and 83.1%, respectively. Dynamic light scattering analysis showed that the DOX-loaded preparations had particle size of less than 300 nm, and could achieve intracellular chemo–photothermal combination therapy by controlling the mesopore on-off based on the gatekeepers, tumor environments including pH and reductant stimuli. The drug-loaded nanoparticles exhibited optimal drug release dynamics in cancer tissues upon triggered by pH and glutathione (GSH) under the NIR irradiation. Cell counting Kit-8 assay manifested that the nanocarriers are non-toxic and safe, whereas the drug-loaded preparations exhibited robust anticancer efficacy. Cellular uptake and TUNEL assay revealed high concentrations of DOX accumulating around and inside Hela cells, accordingly producing significant tumor cell apoptosis. Therefore, the newly-developed materials can be used for comprehensive therapy of cancer as a promising candidate of drug delivered carriers.

通过介孔二氧化硅纳米颗粒接枝聚涂覆一种新颖的中空聚吡咯（2-（4- formylbenzoyloxy）乙基甲基丙烯酸酯共-2-（二甲基氨基）乙基甲基丙烯酸酯） -嵌段-聚（三甘醇甲基醚甲基丙烯酸酯）的多官能纳米复合材料（HPPy @ MSN- SS-NH- g -P(FBEMA- co -DMAEMA)- b -PTEGMA) 的设计和制备是通过二硫键连接。这种作为看门人的材料集成了孔盖、pH 值和高温多刺激响应能力等多功能性，并具有良好的光热稳定性、近红外 (NIR) 光热转换能力和在水溶液中的分散稳定性。它可以通过 HPPy@MSN 的孔通道、两亲共聚物胶束和希夫碱键的核心来捕获抗癌阿霉素（DOX），分别提供高达约 41.5% 和 83.1% 的负载能力和负载效率。动态光散射分析表明，载有 DOX 的制剂粒径小于 300 nm，可以通过控制介孔开关、肿瘤环境（包括 pH 值和还原剂刺激）来实现细胞内化学光热联合治疗。在近红外辐射下，由 pH 值和谷胱甘肽 (GSH) 触发后，载药纳米颗粒在癌组织中表现出最佳的药物释放动力学。细胞计数 Kit-8 分析表明纳米载体无毒且安全，而载药制剂则表现出强大的抗癌功效。细胞摄取和 TUNEL 检测显示，在 Hela 细胞周围和内部积累了高浓度的 DOX，从而产生了显着的肿瘤细胞凋亡。因此，新开发的材料可作为药物递送载体的有希望的候选者，用于癌症的综合治疗。而载药制剂表现出强大的抗癌功效。细胞摄取和 TUNEL 检测显示，在 Hela 细胞周围和内部积累了高浓度的 DOX，从而产生了显着的肿瘤细胞凋亡。因此，新开发的材料可作为药物递送载体的有希望的候选者，用于癌症的综合治疗。而载药制剂表现出强大的抗癌功效。细胞摄取和 TUNEL 检测显示，在 Hela 细胞周围和内部积累了高浓度的 DOX，从而产生了显着的肿瘤细胞凋亡。因此，新开发的材料可作为药物递送载体的有希望的候选者，用于癌症的综合治疗。