当前位置: X-MOL 学术Biochem. Biophys. Res. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
SARS-CoV-2 triggers DNA damage response in Vero E6 cells
Biochemical and Biophysical Research Communications ( IF 2.5 ) Pub Date : 2021-09-15 , DOI: 10.1016/j.bbrc.2021.09.024
Joshua Victor 1 , Jamie Deutsch 1 , Annalis Whitaker 2 , Erica N Lamkin 1 , Anthony March 1 , Pei Zhou 3 , Jason W Botten 4 , Nimrat Chatterjee 5
Affiliation  

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for the current COVID-19 pandemic and has now infected more than 200 million people with more than 4 million deaths globally. Recent data suggest that symptoms and general malaise may continue long after the infection has ended in recovered patients, suggesting that SARS-CoV-2 infection has profound consequences in the host cells. Here we report that SARS-CoV-2 infection can trigger a DNA damage response (DDR) in African green monkey kidney cells (Vero E6). We observed a transcriptional upregulation of the Ataxia telangiectasia and Rad3 related protein (ATR) in infected cells. In addition, we observed enhanced phosphorylation of CHK1, a downstream effector of the ATR DNA damage response, as well as H2AX. Strikingly, SARS-CoV-2 infection lowered the expression of TRF2 shelterin-protein complex, and reduced telomere lengths in infected Vero E6 cells. Thus, our observations suggest SARS-CoV-2 may have pathological consequences to host cells beyond evoking an immunopathogenic immune response.

更新日期:2021-09-30
down
wechat
bug