We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.

中文翻译：

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用于多光子显微镜的基于飞秒二极管的时间透镜激光器

我们展示了一种用于双光子显微镜的具有 kW 峰值功率的基于近红外、飞秒、二极管激光器的光源。该系统在 976 nm 波长下产生亚 ps 脉冲，重复频率为 10 MHz，千瓦级峰值功率适用于深部组织双光子显微镜。该系统与激光扫描显微镜集成，在 PLP-eGFP 标记的小鼠脑组织的固定样本中成像 900 µm 的深度。这代表了一项重大发展，它将为生物医学成像带来更高效、更紧凑和更易于使用的激光源。