Artificial ligands that recognize membrane receptors with high affinity and specificity provide a means for the precise control of receptor activity and the resulting biological functions. In the present work, we focused on a previously reported DNA aptamer that is a functional mimic of fibroblast growth factor (FGF) and evaluated its receptor specificity. It was revealed that the aptamer works as a selective agonist for FGF receptor (FGFR) 1 owing to its high specificity toward the receptor, despite the high sequence homology in the extracellular domains of the FGFR family. The excellent receptor-type specificity of the aptamer, which is distinct from that of native ligands, should offer new possibilities as a chemical tool to study the biological role of growth factor receptors.

中文翻译：

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对成纤维细胞生长因子受体 1 具有高特异性的 DNA 适体的表征

以高亲和力和特异性识别膜受体的人工配体为精确控制受体活性和由此产生的生物学功能提供了一种手段。在目前的工作中，我们专注于先前报道的 DNA 适体，它是成纤维细胞生长因子 (FGF) 的功能模拟物，并评估了其受体特异性。研究表明，尽管 FGFR 家族的细胞外结构域具有高序列同源性，但由于其对受体的高度特异性，适体可作为 FGF 受体 (FGFR) 1 的选择性激动剂。适体与天然配体不同，其出色的受体类型特异性应该为研究生长因子受体的生物学作用的化学工具提供新的可能性。