A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alpha-fetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab₁) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfO-EDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab₂) (anti-AFP₂ and anti-CEA₂), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis.

一种灵敏的夹心模型免疫测定，设计用于同时电化学测量甲胎蛋白和癌胚抗原处理多标记策略。通过组装乙二胺-MWCNT气凝胶（EDA-CAGs）、AuNPs和捕获抗体（Cp-Ab ₁) 在丝网印刷碳电极 (SPCE) 上。使用傅里叶变换红外 (FT-IR)、扫描电子显微镜 (SEM) 和能量色散 X 射线光谱 (EDX) 分析制备的 EDA-CAG 纹理。在该协议中, AuNPs 装饰 EDA-CAG 携带的硫氨酸 (Thi) 和 AuNPs 装饰 EDA-CAG 携带的番红 O (SfO) (分别表示为 AuNP-Thi-EDA-CAG 和 AuNP-SfO-EDA-CAG)被适当地用作可区分的信号标记，用于连接检测抗体（Ab ₂）（抗 AFP ₂和抗 CEA ₂）， 分别。当存在两种肿瘤抗原时，方波伏安法 (SWV) 扫描显示两个分离良好的信号，每个信号表示一个靶抗原。观察到的结果表明，方波伏安峰值电流与两种分析物的对数浓度在 0.005 至 1.0 ng/mL 范围内表现出良好的线性关系。AFP 和 CEA 的 LoD 分别为 0.0015 和 0.0010 ng/mL（信号/噪声 S/N = 3）。将设计的夹心型免疫传感器应用于实际血清样品分析。