Monitoring of Intracellular Vesicles in Cultured Neurons at Different Growth Stages Using Intracellular Vesicle Electrochemical Cytometry,Electroanalysis

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Monitoring of Intracellular Vesicles in Cultured Neurons at Different Growth Stages Using Intracellular Vesicle Electrochemical Cytometry
Electroanalysis ( IF 2.7 ) Pub Date : 2021-09-14 , DOI: 10.1002/elan.202100343
Yueyue Zhang ₁ , Jiangbo Liu ₁ , Xinxin Jing ₁ , Fan Li ₁ , Xiuhai Mao ₁ , Min Li ₁

Affiliation

The exploration of intracellular vesicles plays crucial roles for neuronal activity assessment. Neurons at different growth stages may possess distinct neuronal activity, including vesicular content and release kinetics. Here, we monitored the vesicular content and its release kinetics in neurons at different growth stages by intracellular vesicle electrochemical cytometry. We found that the neurotransmitters content of vesicles changed to be increased and the vesicle release process became longer as the neurons grew. Further, we demonstrated that the vesicular adsorption and rupture modes changed from the dominant simple event to simple and complex event coexisting mode. We speculate that this work provides a new strategy for the neuronal activity assessment or real- time cell activity analysis.

中文翻译：

使用细胞内囊泡电化学流式细胞仪监测不同生长阶段培养的神经元的细胞内囊泡

细胞内囊泡的探索对于神经元活动评估起着至关重要的作用。不同生长阶段的神经元可能具有不同的神经元活动，包括囊泡含量和释放动力学。在这里，我们通过细胞内囊泡电化学细胞术监测了不同生长阶段神经元中的囊泡含量及其释放动力学。我们发现囊泡的神经递质含量随着神经元的生长而增加，囊泡释放过程变长。此外，我们证明了囊泡吸附和破裂模式从主要的简单事件转变为简单和复杂事件共存模式。我们推测这项工作为神经元活动评估或实时细胞活动分析提供了一种新策略。

更新日期：2021-09-14

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