Carboxymethylated cellulose nanofibrils (CNF) with different carboxyl contents (0, 0.36, 0.72 and 1.24 mmol/g) were prepared and characterized via morphology, diameter distribution, zeta potential, structural features, rheological properties, suspension stability, and thermal properties. The results of toxicological studies of ingested CNF via in vitro and in vivo models were present. In vitro studies used an epithelial-like cell line (Caco-2) to assess the effects of a 24 h incubation with CNF, in which no significant cytotoxicity was observed. In vivo studies were evaluated in mice gavage once per day for 8 weeks with 1% or 3.5% w/w suspension of CNF in water. Blood and serum were collected for analysis. No significant differences in hematology, and serum markers were observed between controls and mice given CNF suspensions. Weight, food intake and feces were recorded for growing development and nutrient retention in feces was measured for investigation of functional properties of CNFs. Mice given CNF suspensions gained a significant increment in fecal fat but a reduction in food intake and weight compared to controls. These findings suggested that CNFs are non-toxic and have potentials in behaving as food additives or supplements to reduce caloric intake.

制备了具有不同羧基含量（0、0.36、0.72 和 1.24 mmol/g）的羧甲基化纤维素纳米原纤维 (CNF)，并通过形态、直径分布、zeta 电位、结构特征、流变性能、悬浮稳定性和热性能进行表征。存在通过体外和体内模型对摄入的 CNF进行毒理学研究的结果。体外研究使用上皮样细胞系 (Caco-2) 来评估与 CNF 孵育 24 小时的效果，其中未观察到显着的细胞毒性。体内研究是在小鼠中每天灌胃一次，持续 8 周，其中含有 1% 或 3.5% w/w 的 CNF 悬浮液。收集血液和血清用于分析。在给予 CNF 悬浮液的对照组和小鼠之间没有观察到血液学和血清标志物的显着差异。记录体重、食物摄入量和粪便以促进生长发育，并测量粪便中的营养保留以研究 CNF 的功能特性。与对照组相比，给予 CNF 悬浮液的小鼠粪便脂肪显着增加，但食物摄入量和体重减少。这些发现表明，CNFs 是无毒的，并且具有作为食品添加剂或补充剂来减少热量摄入的潜力。