Dunaliella bardawil, a unicellular green alga, can accumulate a large amount of lutein and β-carotene under stresses. Using chemical inducers combined with abiotic stress to promote the accumulation of high value-added products such as lipids and carotenoids in microalgae has attracted more and more attention. In this study, creatinine was added into autotrophic medium to investigate its effects on the growth, chlorophyll content, and the ingredients and content of carotenoids in D. bardawil. The results showed that creatinine alone could significantly increase the biomass, chlorophyll and carotenoid contents of D. bardawil, among which the contents of lutein and β-carotene were further increased, while the content of zeaxanthin was decreased. In order to further improve the content of the two carotenoids, different light intensities combined with creatinine have been adopted. Under 6.589 W/m² light intensity, creatinine could effectively increase the production of lutein, zeaxanthin, α-carotene and β-carotene. Compared with the control, the content of lutein increased by 46 % and the content of β-carotene increased by 77 % when the concentration of creatinine was 500 1/4g/mL. In conclusion, creatinine can effectively improve the production lutein and β-carotene in D. bardawil, which is more conducive under lower light intensity.

Dunaliella bardawil是一种单细胞绿藻，在压力下可以积累大量的叶黄素和β-胡萝卜素。利用化学诱导剂结合非生物胁迫促进微藻类脂类、类胡萝卜素等高附加值产品的积累越来越受到重视。本研究在自养培养基中加入肌酐，研究其对D. bardawil生长、叶绿素含量以及类胡萝卜素成分和含量的影响。结果表明，单独使用肌酐可显着提高D. bardawil的生物量、叶绿素和类胡萝卜素含量,其中叶黄素和β-胡萝卜素含量进一步升高,而玉米黄质含量降低。为了进一步提高两种类胡萝卜素的含量，采用了不同光强结合肌酐。在6.589 W/m ²光照强度下，肌酐可有效增加叶黄素、玉米黄质、α-胡萝卜素和β-胡萝卜素的产生。与对照组相比，当肌酐浓度为500 1/4g/mL时，叶黄素含量增加46%，β-胡萝卜素含量增加77%。总之，肌酸酐能有效地提高生产叶黄素和β胡萝卜素在D.拜尔代维勒，这是较低的光强度下，更有利于。