Since Antarctica and the surrounding Southern Ocean are facing global climate change, biota inhabiting those coastal regions is now challenged by environmental fluctuations including coastal freshening. In this study, the effects of salinity range of 0–75 (practical salinity unit, PSU) on the Antarctic harpacticoid copepod Tigriopus kingsejongensis was investigated by measurement of 96 h survival rate, lifespan, and sex ratio with further analysis of multigenerational growth parameters and mRNA expressions under salinity of 15–45. Different stages of the copepods (i.e., nauplius, male, and female) generally showed tolerance to hypo– and hypersalinity, wherein female copepods were more tolerant than males when exposed to salinity fluctuations. Lifespan was significantly shortened by hypo- and hypersalinity compared to control salinity (34), but there was no significant difference in the sex ratio between salinity treatments. Multigenerational experiments across five generations revealed that exposure to salinities of 15 and 45 reduced body length compared to that in control salinity and the first generation of each salinity group. Our results provide evidence regarding T. kingsejongensis on their preferred salinity ranges, physiological limit to salinity fluctuations, and population dynamics in future salinity.

由于南极洲和周围的南大洋正面临全球气候变化，居住在这些沿海地区的生物群现在受到包括沿海清新在内的环境波动的挑战。在本研究中，盐度范围 0-75（实际盐度单位，PSU）对南极 harpacticoid 桡足类Tigropus kingsejongensis的影响通过测量 96 小时存活率、寿命和性别比来研究，并进一步分析在 15-45 盐度下的多代生长参数和 mRNA 表达。桡足类的不同阶段（即无节幼体、雄性和雌性）通常表现出对低盐和高盐的耐受性，其中雌性桡足类在暴露于盐度波动时比雄性更耐受。与对照盐度相比，低盐度和高盐度的寿命显着缩短（34），但盐度处理之间的性别比没有显着差异。跨越五代的多代实验表明，与对照盐度和每个盐度组的第一代相比，暴露于 15 和 45 盐度的盐度会缩短体长。我们的结果提供了关于T. kingsejongensis的首选盐度范围、盐度波动的生理限制以及未来盐度的种群动态。