Abstract

Green synthesis of silver nanoparticles (AgNPs) was performed using extracts from Ornithogalum narbonense L. (ONL) as a cost-effective and eco-friendly approach. A surface plasmon resonance peak was observed at 440 nm after 12 h. The intense reddish-brown coloration was observed higher than 1.5 mM AgNO₃ concentrations. Bioactive compounds were found responsible for the reduction, stabilization, and capping of Ag⁺ to AgNPs. X-ray powder diffraction analysis confirmed the face-centered cubic silver crystalline structure. The average spherical-shaped individual particle size was around 8.46 nm, while hydrodynamic size ranged between 7–13 nm and 36–131 nm. The AgNPs have good stability (ζ = –31.15 mV). Energy-dispersive X-ray spectroscopy analysis proved the presence of elemental Ag (6.76%). The antibacterial activity with the zone of inhibition is as follows: P. aeruginosa > E. coli > S.aureus > B. cereus (10.35 ± 0.12 mm > 9.40 ± 0.14 mm > 8.20 ± 0.10 mm > 7.0 ± 0.24 mm). The AgNPs did not show antifungal properties against C. albicans.

• HIGHLIGHTS

• The aqueous extract of Ornithogalum Narbonense L was used for the green synthesis of AgNPs.

• XRD, SEM, EDX, FTIR, ζ-potential, DLS and UV-vis characterizations were made.

• The average surface charge and diameter of individual AgNPs were determined as –31.15 mV and 8.46 nm.

• AgNPs acted as an inhibitor toward E.coli, P.aeruginosa, E.faecalis, S.aureus and B.cereus.

摘要

银纳米粒子 (AgNPs) 的绿色合成是使用从Ornithogalum narbonense L. (ONL) 中提取的一种经济高效且环保的方法。12 小时后在 440 nm 处观察到表面等离子共振峰。观察到高于 1.5 mM AgNO ₃浓度的强烈红棕色着色。发现生物活性化合物负责减少、稳定和封端 Ag ⁺到 AgNPs。X射线粉末衍射分析证实了面心立方银晶体结构。平均球形单个粒径约为 8.46 nm，而流体动力学尺寸范围为 7-13 nm 和 36-131 nm。AgNPs 具有良好的稳定性 (ζ = –31.15 mV)。能量色散 X 射线光谱分析证明元素 Ag (6.76%) 的存在。具有抑制区的抗菌活性如下：铜绿假单胞菌>大肠杆菌>金黄色葡萄球菌>蜡状芽孢杆菌（10.35±0.12毫米>9.40±0.14毫米>8.20±0.10毫米>7.0±0.24毫米）。AgNPs 对白色念珠菌没有显示出抗真菌特性。

• 强调

• Ornithogalum Narbonense L的水提取物用于 AgNPs 的绿色合成。

• 进行了XRD、SEM、EDX、FTIR、ζ-电位、DLS和UV-vis表征。

• 单个 AgNP 的平均表面电荷和直径确定为 –31.15 mV 和 8.46 nm。

• AgNPs 作为大肠杆菌、铜绿假单胞菌、粪肠球菌、金黄色葡萄球菌和蜡状芽胞杆菌的抑制剂。