A novel method combining microsampling of whole blood containing drugs of abuse with 96-well liquid solution microextraction (Parallel Artificial Liquid Membrane Extraction, PALME) and analysis by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was evaluated. Different donor solutions, supported liquid membranes and acceptor solutions were tested, and the most promising set-up was validated. The method included common classes of drugs of abuse, such as opioids, amphetamines, cocaine, benzodiazepines and z-hypnotics. Extraction recoveries above 70% were found for 13 of the compounds, while the last four compounds had recoveries of 10–58%. THC was not extracted with the current method. A linear calibration model was found for all drugs but morphine. Limits of quantitation were between 1 and 5 ng/mL and inter-day precision and accuracy was within 20% for all compounds except for morphine and zopiclone that had a CV of 25% at LOQ. All matrix effects were within 78–123%. Samples were stable for 14 days except for zopiclone and zolpidem. With low-cost, high sample throughput, semi-automated miniaturized sample preparation in combination with dried blood microsamples, and an Eco-Scale score of 78, the proposed method fulfills green chemistry principles.

一种将含有滥用药物的全血微量采样与96孔液体溶液微萃取（平行人工液膜萃取）相结合的新方法, PALME) 和超高效液相色谱-串联质谱 (UHPLC-MS/MS) 分析进行了评估。测试了不同的供体溶液、支持的液体膜和受体溶液，并验证了最有希望的设置。该方法包括常见的滥用药物类别，如阿片类药物、安非他明、可卡因、苯二氮卓类和 z-催眠药。发现其中 13 种化合物的萃取回收率高于 70%，而最后四种化合物的回收率为 10-58%。目前的方法未提取 THC。为除吗啡之外的所有药物找到了线性校准模型。定量限在 1 到 5 ng/mL 之间，所有化合物的日间精密度和准确度均在 20% 以内，但在 LOQ 下的 CV 为 25% 的吗啡和佐匹克隆除外。所有基质效应都在 78-123% 之内。除佐匹克隆和唑吡坦外，样品稳定 14 天。该方法成本低、样品通量高、半自动化小型化样品制备与干血微量样品相结合，生态评分为 78，符合绿色化学原则。