Dynamic motions and architectural changes in DNA supramolecular aggregates visualized via transmission electron microscopy without liquid cells,Nanoscale

当前位置： X-MOL 学术 › Nanoscale › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Dynamic motions and architectural changes in DNA supramolecular aggregates visualized via transmission electron microscopy without liquid cells
Nanoscale ( IF 5.8 ) Pub Date : 2021-09-07 , DOI: 10.1039/d1nr04133a
Zhuoyang Lu ₁ , Xiangyang Liu ₂ , Maogang He ₂ , Jiangang Long ₁ , Jiankang Liu ₁

Affiliation

In the last decade, breakthroughs in liquid-phase transmission electron microscopy (TEM) have enabled in situ visualization of the motion dynamics of nanostructures in liquid media with unprecedented detail. However, it remains a significant challenge to perform liquid-phase TEM due to the intricate preparation procedure of liquid cells to keep liquid from evaporating under ultrahigh vacuum conditions in TEM columns. In the present study, the nonvolatility and remarkable solvation property of ionic liquids (ILs) is exploited to image the dynamic processes of DNA supramolecular aggregates and Au nanoparticle (NP) aggregates encompassing Brownian motions, interactions among individual nanoobjects and changes in architecture at nanometer resolution. Significant differences in motion behaviors are observed between DNA supramolecular aggregates and Au NP aggregates. Moreover, the temperature and dose dependence of dynamic motions are also investigated. The findings provide insights into the dynamics of DNA supramolecular aggregates and Au NP aggregates in ILs and present an easily accessible approach for probing the dynamic processes of biomacromolecular and other soft matter aggregates with various kinds of ILs at the nanoscale level.

中文翻译：

通过无液体细胞的透射电子显微镜观察 DNA 超分子聚集体的动态运动和结构变化

在过去十年中，液相透射电子显微镜 (TEM) 的突破使原位以前所未有的细节可视化液体介质中纳米结构的运动动力学。然而，由于液体细胞的复杂制备过程以防止液体在 TEM 柱中的超高真空条件下蒸发，因此进行液相 TEM 仍然是一个重大挑战。在本研究中，利用离子液体 (IL) 的非挥发性和显着的溶剂化特性，对 DNA 超分子聚集体和 Au 纳米粒子 (NP) 聚集体的动态过程进行成像，包括布朗运动、单个纳米物体之间的相互作用以及纳米分辨率下的结构变化. 在 DNA 超分子聚集体和 Au NP 聚集体之间观察到运动行为的显着差异。此外，还研究了动态运动的温度和剂量依赖性。

更新日期：2021-09-15

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11