Engineered multifunctional metal–phenolic nanocoatings for label-free capture and “self-release” of heterogeneous circulating tumor cells,Nanoscale

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Engineered multifunctional metal–phenolic nanocoatings for label-free capture and “self-release” of heterogeneous circulating tumor cells
Nanoscale ( IF 5.8 ) Pub Date : 2021-08-23 , DOI: 10.1039/d1nr04112f
Yiling Liu ₁ , Xiaoshan Wang ₂ , Yuwei Zhou ₁ , Guang Yang ₃ , Jianwen Hou ₁ , Shaobing Zhou ₁

Affiliation

Immunomagnetic beads have been widely explored as an important analytical tool for the rapid and sensitive detection of circulating tumor cells (CTCs). However, their clinical application is seriously hindered by the tedious preparation procedures and heterogeneous nature of CTCs. To this end, a designed multifunctional platform named Fe₃O₄@TA/Cu^II superparamagnetic nanoparticles (SPMNPs) is expected to have the following features: (i) the formation of a tannic acid–copper (II) ion (TA/Cu^II) coating which could be accomplished by a one-step method is very simple; (ii) the TA/Cu^II coating shows high affinity for heterogeneous CTCs and good resistance to nonspecific adhesion of blood cells; (iii) “self-release” of the captured cells could be achieved as the TA/Cu^II coating gradually degrades in the cell culture environment without any additional interventions. Therefore, the resulting Fe₃O₄@TA/Cu^II SPMNPs could capture various CTCs (MCF-7, HepG2 and HeLa cells) with different expression levels of the epithelial cell adhesion molecule (EpCAM). And the capture efficiency and cell purity can reach 88% and 87%, respectively. In addition, 68% of the captured cells are self-released after 6 h of incubation and most of the released cells show high cell proliferation activity. In particular, Fe₃O₄@TA/Cu^II SPMNPs can successfully detect 1–13 CTCs from 1 mL of blood of 14 patients with 6 types of cancers. Hence, we expect that the as-prepared Fe₃O₄@TA/Cu^II SPMNPs with simple, efficient, and universal yet cost-efficient characteristics could act as a promising analytical tool for clinical CTC detection.

中文翻译：

用于无标记捕获和“自释放”异质循环肿瘤细胞的多功能金属酚纳米涂层

免疫磁珠作为一种重要的分析工具被广泛探索，用于快速、灵敏地检测循环肿瘤细胞 (CTC)。然而，它们的临床应用受到 CTC 繁琐的制备程序和异质性的严重阻碍。为此，设计的多功能平台 Fe ₃ O ₄ @TA/Cu ^II超顺磁性纳米粒子 (SPMNPs) 有望具有以下特征：(i) 单宁酸-铜 ( II ) 离子 (TA/Cu ) 的形成^II ) 一步法即可完成的包衣非常简单；(ii) TA/Cu ^II涂层对异质 CTC 具有高亲和力，对血细胞的非特异性粘附具有良好的抵抗力；(iii) 由于 TA/Cu ^II涂层在细胞培养环境中逐渐降解而无需任何额外干预，因此可以实现捕获细胞的“自我释放” 。因此，由此产生的 Fe ₃ O ₄ @TA/Cu ^II SPMNPs 可以捕获具有不同表达水平的上皮细胞粘附分子 (EpCAM) 的各种 CTC（MCF-7、HepG2 和 HeLa 细胞）。并且捕获效率和细胞纯度可以分别达到88%和87%。此外，68% 的捕获细胞在孵育 6 小时后自我释放，并且大部分释放的细胞显示出高细胞增殖活性。特别是 Fe ₃O ₄ @TA/Cu ^II SPMNPs 可以从 14 名患有 6 种癌症的患者的 1 mL 血液中成功检测到 1-13 个 CTC。因此，我们期望所制备的 Fe ₃ O ₄ @TA/Cu ^II SPMNPs 具有简单、高效、通用且具有成本效益的特性，可以作为临床 CTC 检测的有前途的分析工具。

更新日期：2021-09-15

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