Continuous multicolumn Protein A chromatography offers various advantages for capture stage purification of monoclonal antibody therapeutics, like higher productivity and resin capacity utilization, lower buffer consumption, small footprint, etc. Due to the complexity of the continuous process, experimental optimization is time-consuming and cost-intensive. This investigation proposes a hybrid process development approach integrating experimental and mechanistic modeling for time- and cost-effective development and optimization of continuous Protein A affinity chromatography.

中文翻译：

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生物仿制药单克隆抗体治疗剂连续捕获步骤纯化的机理建模

连续多柱蛋白 A 层析为单克隆抗体治疗药物的捕获阶段纯化提供了各种优势，如更高的生产率和树脂容量利用率、更低的缓冲液消耗、小占地面积等。由于连续过程的复杂性，实验优化耗时且成本密集。本研究提出了一种混合工艺开发方法，将实验和机械建模相结合，以实现连续蛋白 A 亲和层析的时间和成本效益开发和优化。