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Campylobacter concisus upregulates PD-L1 mRNA expression in IFN-γ sensitized intestinal epithelial cells and induces cell death in esophageal epithelial cells
Journal of Oral Microbiology ( IF 3.7 ) Pub Date : 2021-09-14 , DOI: 10.1080/20002297.2021.1978732
Seul A Lee 1 , Fang Liu 1 , Doo Young Yun 1 , Stephen M Riordan 2 , Alfred Chin Yen Tay 3 , Lu Liu 4 , Cheok Soon Lee 5, 6, 7, 8 , Li Zhang 1
Affiliation  

ABSTRACT

Introduction: Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) and Barrett’s esophagus (BE). Programmed cell death ligand-1 (PD-L1) is an immune checkpoint protein that is used by tumor cells for immune evasion and has increased expression in patients with IBD and BE. We examined whether C. concisus upregulates PD-L1 expression in intestinal and esophageal epithelial cells.

Methods: Human intestinal epithelial HT-29 cells and esophageal epithelial FLO-1 cells with and without interferon (IFN)-γ sensitization were incubated with C. concisus strains. The level of PD-L1 mRNA was quantified using quantitative real-time PCR. Cytokines were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Apoptosis of HT-29 and FLO-1 cells were measured using caspase 3/7 assay.

Results: We found that intestinal epithelial cells with IFN-γ sensitization incubated with C. concisus significantly upregulated PD-L1 expression and significantly increased the production of interleukin (IL)-8. Whereas, PD-L1 expression was significantly inhibited in IFN-γ sensitized FLO-1 cells incubated with C. concisus strains. Furthermore, FLO-1 cells with and without IFN-γ sensitization incubated with C. concisus strains both had significantly higher levels of cell death.

Conclusion: C. concisushas the potential to cause damage to both intestinal and esophageal epithelial cells, however, with different pathogenic effects.



中文翻译:


弯曲杆菌上调 IFN-γ 致敏肠上皮细胞中 PD-L1 mRNA 表达并诱导食管上皮细胞死亡


 抽象的


简介: Conpylobacter concisus是一种口腔细菌,与炎症性肠病 (IBD) 和巴雷特食管 (BE) 相关。程序性细胞死亡配体-1 (PD-L1) 是一种免疫检查点蛋白,肿瘤细胞利用它来逃避免疫,并且在 IBD 和 BE 患者中表达增加。我们检查了C. concisus是否上调肠和食管上皮细胞中的 PD-L1 表达。


方法:将有或没有干扰素(IFN)-γ致敏的人肠上皮HT-29细胞和食管上皮FLO-1细胞与C. concisus菌株一起孵育。使用定量实时 PCR 对 PD-L1 mRNA 水平进行定量。使用酶联免疫吸附测定(ELISA)测量细胞因子。使用 caspase 3/7 测定法测量 HT-29 和 FLO-1 细胞的凋亡。


结果:我们发现,用 IFN-γ 致敏的肠上皮细胞与C. concisus一起孵育,显着上调 PD-L1 表达,并显着增加白细胞介素 (IL)-8 的产生。然而,在与C. concisus菌株一起孵育的 IFN-γ 致敏 FLO-1 细胞中,PD-L1 表达显着受到抑制。此外,与C. concisus菌株一起孵育的具有和不具有 IFN-γ 致敏作用的 FLO-1 细胞均具有显着更高水平的细胞死亡。


结论: C. concisus有可能对肠和食管上皮细胞造成损害,但致病作用不同。

更新日期:2021-09-15
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