Dispersin B (DspB) is a member of glycoside hydrolase family 20 (GH20) and catalyzes degradation of biofilms forming by pathogenic bacteria such as Staphylococcus aureus. Magnetoreceptor (MagR) is a magnetic protein that can be used as a fusion partner for functionally immobilizing proteins on magnetic surfaces. In the present study, a recombinant protein DspB-MagR was constructed by fusing MagR to the C-terminus of DspB and expressed in Escherichia coli. Magnetic immobilization of purified DspB-MagR on magnetic core–shell structured Fe₃O₄@SiO₂ nanoparticles was achieved and characterized by means of various techniques including SDS-PAGE, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential measurement, and scanning electron microscopy. It was evaluated the influence of temperature, pH, and storage time on the performance of immobilized DspB-MagR on Fe₃O₄@SiO₂ nanoparticles. Removal of biofilms forming by Staphylococcus aureus and other medical sourced bacterial species was achieved by using Fe₃O₄@SiO₂ nanoparticles loading with DspB-MagR. This work promoted potential applications of DspB and similar enzymes for medical purposes.

Dispersin B (DspB) 是糖苷水解酶家族 20 (GH20) 的成员，可催化由金黄色葡萄球菌等病原菌形成的生物膜的降解。磁感受器（Magnetoreceptor，Magnetoreceptor，Magnetoreceptor）是一种磁性蛋白质，可用作融合伴侣，将蛋白质功能性固定在磁性表面上。本研究通过将MagR与DspB的C端融合构建重组蛋白DspB-MagR，并在大肠杆菌中表达。纯化的 DspB-MagR 在磁性核壳结构 Fe ₃ O ₄ @SiO _{2上的磁性固定}纳米粒子是通过各种技术实现和表征的，包括 SDS-PAGE、傅里叶变换红外光谱、热重分析、zeta 电位测量和扫描电子显微镜。评估了温度、pH 和储存时间对固定化 DspB-MagR 在 Fe ₃ O ₄ @SiO ₂纳米粒子上的性能的影响。通过使用负载有 DspB-MagR的 Fe ₃ O ₄ @SiO ₂纳米颗粒去除由金黄色葡萄球菌和其他医学来源的细菌物种形成的生物膜。这项工作促进了 DspB 和类似酶在医疗方面的潜在应用。