Carbapenem-resistant Acinetobacter baumannii (CRAB) has been a common pathogen of nosocomial infections and severely threatened the public health for decades. Tigecycline is a new type of antibacterial glycylcycline and minocycline derivative and has been used to treat CRAB in clinical practice. However, the synergistic effects of tigecycline in combination with other antibiotics including colistin or amikacin remain unclear. A total of 216 CRAB isolates were collected from multiple body parts of different patients. The gene types of these isolates were analyzed and their resistance to carbapenems was determined by Etest. Broth microdilution method was utilized to evaluate the minimum inhibitory concentration (MIC) of each sample. Checkerboard screening technique was performed to demonstrate the synergistic effects of antibiotics and fractional inhibitory concentration index (FICI) was established. Therefore, the joint treatment of tigecycline and colistin (1:1) could effectively improve the sensitivity of AB to antibiotics. OXA-24-like isolates were more sensitive to the combination of tigecycline and amikacin. On the other hand, OXA-23-like isolates were more sensitive to the combination of tigecycline and colistin. Tigecycline exhibited synergistic effects with amikacin and colistin to inhibit CRAB.

耐碳青霉烯类鲍曼不动杆菌(CRAB) 一直是医院感染的常见病原体，几十年来严重威胁公众健康。替加环素是一种新型抗菌甘氨酰环素和米诺环素衍生物，临床已用于治疗CRAB。然而，替加环素与包括粘菌素或阿米卡星在内的其他抗生素联合使用的协同作用仍不清楚。从不同患者的多个身体部位共收集了 216 株 CRAB 分离株。分析了这些分离株的基因类型，并通过Etest确定了它们对碳青霉烯类的抗性。肉汤微量稀释法用于评估每个样品的最小抑菌浓度（MIC）。进行棋盘筛选技术以证明抗生素的协同作用并建立分数抑制浓度指数（FICI）。因此，替加环素与粘菌素（1：1）联合治疗可有效提高AB对抗生素的敏感性。OXA-24 样分离株对替加环素和阿米卡星的组合更敏感。另一方面，OXA-23 样分离物对替加环素和粘菌素的组合更敏感。替加环素与阿米卡星和多粘菌素具有协同抑制 CRAB 的作用。OXA-23 样分离株对替加环素和粘菌素的组合更敏感。替加环素与阿米卡星和多粘菌素具有协同抑制 CRAB 的作用。OXA-23 样分离株对替加环素和粘菌素的组合更敏感。替加环素与阿米卡星和多粘菌素具有协同抑制 CRAB 的作用。