Mineral stress is one of the major abiotic stresses faced by crop plants. The present study was undertaken to investigate the impact of mineral stress (iron (Fe) and phosphorus (P)) on various morphological and biochemical responses of the shoot and root tissues and root architecture of common bean (Phaseolus vulgaris L.). This study also leads us to the identification of P stress responsive proteins. The study was conducted under in vitro conditions, in which seeds of Shalimar French Bean-1 (SFB-1) variety were cultured on four different MGRL medium (control (P₁Fe₁), iron deficient (P₁Fe₀), phosphorus deficient (P₀Fe₁), and phosphorus and iron deficient (P₀Fe₀)). Chlorophyll content of leaves, Fe/P content of root tissues, total sugars, proline, length, and weight of shoot and root tissues were assessed and compared within and between the treatments. The analyzed data revealed significant difference between control and other three treatments. Chlorophyll content of shoots was found significantly decreased under mineral stress treatments P₀Fe₁, P₁Fe₀, and P₀Fe₀ than control. Length and weight of shoot and root were also observed significantly decreased under P₀Fe₁, P₁Fe₀, and P₀Fe₀ as compared to control. Total sugar was significantly higher in P₀Fe₁ of roots in comparison to control. Proline content was significantly higher in both tissues of shoots and roots of plants grown under P₁Fe₀, P₀Fe₁, and P₀Fe₀ than control condition. Furthermore, we unexpectedly observed the recovery of roots (mainly primary roots) under P₀Fe₀ as compared to P₁Fe₀ and P₀Fe₁^. Interestingly higher concentration of Fe was also observed in P₀Fe₁ compared to other treatments and also higher concentration of P was observed in P₁Fe₁. These findings suggested that there is a crosstalk between Fe and P and also revealed that there is a disruption in the ability of PR (primary root) to sense local P deficiency in the absence of Fe. Furthermore, proteomics analysis (SDS-PAGE followed by MALDI MS) helped in identification of defensive proteins in P stress condition compared to control.

矿物胁迫是农作物面临的主要非生物胁迫之一。本研究旨在调查矿物胁迫（铁 (Fe) 和磷 (P)）对菜豆 ( Phaseolus vulgaris L.) 枝条和根组织以及根结构的各种形态和生化反应的影响。这项研究还引导我们鉴定 P 应激反应蛋白。该研究是在体外条件下进行的，其中 Shalimar French Bean-1 (SFB-1) 品种的种子在四种不同的 MGRL 培养基（对照 (P ₁ Fe ₁ )、缺铁 (P ₁ Fe ₀ )、磷缺乏（P ₀ Fe ₁），缺乏磷和铁（P₀铁₀））。对叶片的叶绿素含量、根组织的铁/磷含量、总糖、脯氨酸、芽和根组织的长度和重量进行了评估，并在处理内和处理间进行了比较。分析数据显示对照和其他三种处理之间存在显着差异。_{矿物胁迫处理P 0} Fe ₁、P ₁ Fe ₀和P ₀ Fe ₀下，地上部叶绿素含量显着低于对照。_{在 P 0} Fe ₁、P ₁ Fe ₀和 P ₀下也观察到茎和根的长度和重量显着降低Fe ₀与对照相比。与对照相比，根的P ₀ Fe ₁中总糖显着升高。_{在P 1} Fe ₀、P ₀ Fe ₁和P ₀ Fe ₀下生长的植物的芽和根组织中脯氨酸含量显着高于对照条件。_{此外，与P 1} Fe ₀和P ₀ Fe ₁相比，我们出乎意料地观察到在P ₀ Fe ₀下根（主要是初生根）的恢复^。有趣的是，与其他处理相比，在 P ₀ Fe _{1中也观察到更高浓度的 Fe，在 P 1} Fe ₁中也观察到更高浓度的P。这些发现表明 Fe 和 P 之间存在串扰，并且还表明 PR（主根）在没有 Fe 的情况下感知局部 P 缺乏的能力受到破坏。此外，与对照相比，蛋白质组学分析（SDS-PAGE，然后是 MALDI MS）有助于鉴定 P 胁迫条件下的防御蛋白。