Lysozyme is widely used for the synthesis of nanomaterials (e.g., gold nanoparticle) to fluorescently sense metal ions. However, the effect of metal ions on the fluorescence of lysozyme is not studied yet. Herein, we have explored the interactions of lysozyme with different metal ions to develop a direct sensing platform for Fe(III). It has been observed that the fluorescence of lysozyme was slightly decreased in the presence of Cu(II), Hg(II), As(V), Co(II), Cd(II), Cr(II), Fe(II), Mn(II), Pb(II), and Zn(II), while a significant decrease in the lysozyme fluorescence was observed for Fe(III). The effect of thermal stability on the fluorescence quenching was also studied from 25 to 60 °C. In the present study, the lysozyme sensing probe was able to selectively and accurately detect 0.5–50 ppm of Fe(III) with a LOD of 0.1 ppm (1.8 µM) at 25 °C.

溶菌酶广泛用于合成纳米材料（例如金纳米颗粒）以荧光感应金属离子。然而，尚未研究金属离子对溶菌酶荧光的影响。在此，我们探索了溶菌酶与不同金属离子的相互作用，以开发一个直接感应 Fe(III) 的平台。已观察到在存在 Cu(II)、Hg(II)、As(V)、Co(II)、Cd(II)、Cr(II)、Fe(II) 的情况下，溶菌酶的荧光略有下降、Mn(II)、Pb(II) 和 Zn(II)，而对 Fe(III) 观察到溶菌酶荧光显着降低。还研究了从 25 到 60 °C 的热稳定性对荧光猝灭的影响。在本研究中，溶菌酶传感探针能够在 25 °C 下选择性且准确地检测 0.5–50 ppm 的 Fe(III)，LOD 为 0.1 ppm (1.8 µM)。