Novel alkylated heterocyclic chalcone (E)-1-(2-(allyloxy)phenyl)-3-(9-ethyl-9H-carbazol-3-yl)prop-2-en-1-one (AECO) with extended π-bond was prepared by the multi-steps synthesis. The structure of the AECO was established by the spectroscopic technics and purity of the compound was confirmed by the elemental analysis. Physicochemical parameters of the AECO such as molar absorption coefficient, transition dipole moments, stokes shift, oscillator strength and fluorescence quantum yield were calculated in ten various solvents on the basis of polarity of the solvents to see the effect of the solvent with AECO. Interaction of the AECO chromophore with cationic CTAB and anionic SDS surfactants were determined by using the fluorescence spectroscopy techniques. The intensity of the florescence spectrum increase with increasing the concentrations of surfactants. This suggests that strong interaction occurs between AECO with surfactants and this interaction arise from electrostatic forces. So, AECO chromophore could be used as analysis to define the Critical Micelle Concentration (CMC) of the surfactants. In addition the in-vitro antibacterial active of novel heterocyclic chalcone agents four bacteria’s strain were evaluated and result showed AECO is beater antibacterial agent against Gram-Negative Bacteria (E. coli and S. flexneri) as compare to the Gram Negative Bacteria with respected to the standard drug Tetracycline.

新型烷基化杂环查尔酮 (E)-1-(2-(烯丙氧基)苯基)-3-(9-乙基-9H-咔唑-3-基)丙-2-en-1-酮 (AECO)，具有扩展的 π-通过多步合成制备了键。通过光谱技术确定了AECO的结构，并通过元素分析确认了化合物的纯度。根据溶剂的极性，计算了10种不同溶剂中AECO的摩尔吸收系数、跃迁偶极矩、斯托克斯位移、振荡强度和荧光量子产率等物理化学参数，以观察溶剂对AECO的影响。使用荧光光谱技术测定 AECO 发色团与阳离子 CTAB 和阴离子 SDS 表面活性剂的相互作用。荧光光谱的强度随着表面活性剂浓度的增加而增加。这表明 AECO 与表面活性剂之间发生了强烈的相互作用，并且这种相互作用是由静电力引起的。因此，AECO 发色团可用于分析来定义表面活性剂的临界胶束浓度 (CMC)。此外，对新型杂环查尔酮剂的体外抗菌活性对四种细菌菌株进行了评估，结果表明，与革兰氏阴性菌相比，AECO 是针对革兰氏阴性菌（大肠杆菌和弗氏链球菌）的最佳抗菌剂。标准药物四环素。