We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, biodegradation rate (k) and half-life (t_1/2) values were found to be 0.038 day⁻¹ and 18.09 days for P. aeruginosa AKS1, and 0.020 day⁻¹ and 33.97 days in case of Bacillus sp. AKS2, respectively. In microcosm sediments, the estimated k and t _1/2 values were 0.014 day⁻¹ and 50 days for P. aeruginosa AKS1, and 0.011 day⁻¹ and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N and 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N and 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, values of k and t_1/2 were found to be 0.007 day⁻¹ and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were 0.005 day⁻¹ and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

我们报告了铜绿假单胞菌 AKS1 和 芽孢杆菌降解阿萨姆原油的动力学  。AKS2，均从阿萨姆炼油厂沉积物中分离出来。分离物表现出明显的疏水性、乳化指数和生物表面活性剂的产生。在 (1) 用 1% v/v 原油和 (2) 微观沉积物（125 毫克原油/10 克沙子）修正的液体介质中评估分离物的原油降解效率 。在液体培养中，铜绿假单胞菌 AKS1的生物降解率 ( k ) 和半衰期 ( t _1/2 ) 值分别为 0.038 天^-1和 18.09 天 ，^如果是 芽孢 杆菌 AKS2，分别。在微观世界沉积物中，估计的k和t  _1/2值对于铜绿假单胞菌 AKS1为 0.014 天^-1和 50 天，对于 芽孢杆菌属为 0.011 天^-1和 61.34 天  。AKS2。在铜绿假单胞菌AKS1的情况下，微宇宙砂沉积物中的养分处理水平为 125 µg N 和 62.5 µg P/g 沉积物， 在芽孢杆菌 的情况下为 375 µg N 和 37.5 µg P/g 沉积物  。AKS2。在没有无机营养素的微观世界中，k和t _1/2的值被发现为 0.007 天^-1和 100 天 铜绿假单胞菌 AKS1 和 芽孢杆菌 。AKS2，分别为0.005天^-1和150.68天。我们的数据为预测液体介质和受污染沉积物中的碳氢化合物降解提供了重要信息。