A wavelength modulation based surface plasmon resonance (SPR) sensor setup was developed with inexpensive components. The developed setup is utilized for the detection of cardiac marker proteins, cardiac troponin I (CTnI), and cardiac troponin T (CTnT). A simple approach was used for the fabrication of CTnI/CTnT antibody sensor chip. A self-assembled monolayer (SAM) of 1-octanethiol and 11-mercaptoundecanoic acid (11-MUA) was formed on annealed Au thin film. The −COOH group of 11-MUA was modified with EDC-NHS for amine coupling to monoclonal antibodies of CTnI and CTnT. The developed SPR setup and the sensor chip with monoclonal antibodies showed good performance for the detection of CTnI and CTnT in direct assay. For CTnI, the lowest concentration detected was 0.03125 ng/mL whereas for CTnT, it was 0.0625 μg/mL. The detected concentrations are well below the onset concentration of these two proteins in blood following the acute myocardial infarction (AMI). The performance of this direct assay based on the developed setup and sensor chip therefore can be used easily for detection of CTnI and CTnT during their onset period.

基于波长调制的表面等离子体共振 (SPR) 传感器装置是用廉价的组件开发的。开发的设置用于检测心脏标记蛋白、心肌肌钙蛋白 I (CTnI) 和心肌肌钙蛋白 T (CTnT)。一种简单的方法用于制造 CTnI/CTnT 抗体传感器芯片。在退火的 Au 薄膜上形成了 1-辛硫醇和 11-巯基十一烷酸 (11-MUA) 的自组装单层 (SAM)。11-MUA 的 -COOH 基团用 EDC-NHS 修饰，用于胺偶联到 CTnI 和 CTnT 的单克隆抗体。开发的 SPR 设置和带有单克隆抗体的传感器芯片在直接测定中检测 CTnI 和 CTnT 显示出良好的性能。对于 CTnI，检测到的最低浓度为 0.03125 ng/mL，而对于 CTnT，检测到的最低浓度为 0.0625 μg/mL。检测到的浓度远低于急性心肌梗塞 (AMI) 后血液中这两种蛋白质的起始浓度。因此，基于开发的设置和传感器芯片的这种直接测定的性能可以很容易地用于检测 CTnI 和 CTnT 在其发病期间。