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Activation of stably silenced genes by recruitment of a synthetic de-methylating module
bioRxiv - Synthetic Biology Pub Date : 2021-09-11 , DOI: 10.1101/2021.09.11.459888
Wing Fuk Chan , Christine R. Keenan , Timothy M Johanson , Rhys S Allan

Stably silenced genes that display a high level of CpG dinucleotide methylation are refractory to the current generation of dCas9-based activation systems. To counter this, we created an improved activation system by coupling the catalytic domain of DNA demethylating enzyme TET1 with transcriptional activators (TETact). TETact induces transcription of heavily suppressed non-coding RNA and surface protein, and the reactivation of embryonic haemoglobin genes in non-erythroid cells.

中文翻译:

通过募集合成去甲基化模块激活稳定沉默的基因

显示出高水平 CpG 二核苷酸甲基化的稳定沉默基因对当前一代基于 dCas9 的激活系统难以处理。为了解决这个问题,我们通过将 DNA 去甲基化酶 TET1 的催化结构域与转录激活因子 (TETact) 偶联,创建了一个改进的激活系统。TETact 诱导严重抑制的非编码 RNA 和表面蛋白的转录,以及非红系细胞中胚胎血红蛋白基因的重新激活。
更新日期:2021-09-14
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