Actin assembly at the hyphal tip is key for polar growth and pathogenesis of the rice blast fungus Magnaporthe oryzae. The mechanism of its precise assemblies and biological functions is not understood. Here, we characterized the role of M. oryzae Twinfilin (MoTwf) in M. oryzae infection through organizing the actin cables that connect to Spitzenkörper (Spk) at the hyphal tip. MoTwf could bind and bundle the actin filaments. It formed a complex with Myosin2 (MoMyo2) and the Woronin body protein Hexagonal peroxisome 1 (MoHex1). Enrichment of MoMyo2 and MoHex1 in the hyphal apical region was disrupted in a ΔMotwf loss-of-function mutant, which also showed a decrease in the number and width of actin cables. These findings indicate that MoTwf participates in the virulence of M. oryzae by organizing Spk-connected actin filaments and regulating MoHex1 distribution at the hyphal tip.

中文翻译：

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Twinfilin 调控肌动蛋白组装和六角过氧化物酶体 1 (Hex1) 定位在稻瘟病真菌稻瘟病菌发病机制中的作用

菌丝末端的肌动蛋白组装是稻瘟病真菌稻瘟病菌极地生长和发病机制的关键。其精确组装和生物学功能的机制尚不清楚。在这里，我们描述了M. oryzae Twinfilin (MoTwf) 在M中的作用。 通过组织在菌丝尖端连接到 Spitzenkörper (Spk) 的肌动蛋白电缆来感染米菌。MoTwf 可以结合和捆绑肌动蛋白丝。它与 Myosin2 (MoMyo2) 和 Woronin 体蛋白 Hexagonal peroxisome 1 (MoHex1) 形成复合物。MoMyo2 和 MoHex1 在菌丝顶端区域的富集在 Δ Motwf中被破坏功能丧失突变体，也显示肌动蛋白电缆的数量和宽度减少。这些发现表明，MoTwf通过组织与 Spk 连接的肌动蛋白丝和调节 MoHex1 在菌丝尖端的分布来参与米霉的毒力。