An aptasensor for the detection of Pb2+ based on photoinduced electron transfer between a G-quadruplex–hemin complex and a fluorophore,Luminescence

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An aptasensor for the detection of Pb2+ based on photoinduced electron transfer between a G-quadruplex–hemin complex and a fluorophore
Luminescence ( IF 3.2 ) Pub Date : 2021-09-13 , DOI: 10.1002/bio.4141
Ya Wang ₁ , Tai Ye ₁ , Min Yuan ₁ , Hui Cao ₁ , Jingsong Yu ₁ , Fengqin Yin ₁ , Xiuxiu Wu ₁ , Liling Hao ₁ , Fei Xu ₁

Affiliation

Due to the threat to health of heavy metal contamination, simple and rapid detection methods for heavy metals are an urgent needed in environment protection and food safety. In this work, we have developed a fluorescent aptasensor for the ‘turn-off’ model detection of Pb²⁺. The key feature of the aptasensor is that the dye-labelled nucleic acid strand can be folded into a G-quadruplex structure in the presence of Pb²⁺. This conformational change induces photoinduced electron transfer (PET) between a G-quadruplex–hemin complex and 6-carboxyrhodamine X (ROX), which results in fluorescence quenching of ROX. We systematically investigated the interaction mechanism between Pb²⁺ and the aptasensor and the effects of several environmental parameters were also studied. Under the optimum conditions, the proposed method exhibited a good liner relationship between the concentration of Pb²⁺ and fluorescence quenching efficiency in the range 25–500 nM and the limit of detection was 1.02 nM. In addition, this method also manifested good performance in spiked lettuce samples with satisfactory recoveries of 87.10–109.6%. This target-induced PET platform can be further expanded to other heavy metal analysis.

中文翻译：

基于 G-四链体-血红素复合物和荧光团之间的光诱导电子转移检测 Pb2+ 的适体传感器

由于重金属污染对健康的威胁，在环境保护和食品安全方面迫切需要简单、快速的重金属检测方法。在这项工作中，我们开发了一种用于 Pb ²⁺的“关闭”模型检测的荧光适体传感器。适体传感器的关键特征是染料标记的核酸链可以在 Pb ²⁺存在下折叠成 G-四链体结构。这种构象变化会诱导 G-四链体-氯化血红素复合物和 6-羧罗丹明 X (ROX) 之间的光诱导电子转移 (PET)，从而导致 ROX 的荧光猝灭。我们系统地研究了 Pb ²⁺之间的相互作用机制还研究了适体传感器和几个环境参数的影响。在最佳条件下，所提出的方法在25-500 nM范围内表现出Pb ²⁺浓度与荧光猝灭效率之间良好的线性关系，检测限为1.02 nM。此外，该方法在加标生菜样品中也表现出良好的性能，回收率为 87.10-109.6%。这种目标诱导的 PET 平台可以进一步扩展到其他重金属分析。

更新日期：2021-09-13

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