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Antioxidant properties and inhibition of lipid formation in 3T3-L1 adipocytes of in vitro digested mageu, a commercial sample
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-09-14 , DOI: 10.1111/jfbc.13929 Haleema Nathu 1 , Kabuzi R Mbuyama 2 , Martin Adarkwah-Yiadom 3 , June C Serem 1 , Mohammed Auwal Ibrahim 4 , Kwaku G Duodu 3 , Anabella R M Gaspar 2 , Megan J Bester 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-09-14 , DOI: 10.1111/jfbc.13929 Haleema Nathu 1 , Kabuzi R Mbuyama 2 , Martin Adarkwah-Yiadom 3 , June C Serem 1 , Mohammed Auwal Ibrahim 4 , Kwaku G Duodu 3 , Anabella R M Gaspar 2 , Megan J Bester 1
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Mageu is a fermented, non-alcoholic maize-derived product unique to southern Africa. The aim of this study was to identify the health benefits of a polyphenolic extract of commercially produced mageu related to the antioxidant properties and effects on lipid accumulation in differentiated 3T3-L1 adipocytes. A pooled sample of mageu Number 1 brand (original non-flavored) was subjected to in vitro gastroduodenal digestion (GDD). Reverse phase high-performance liquid chromatography of unfractionated undigested (UD) and GDD mageu revealed that with digestion there was an increased extraction of 1.2, 1.83, 1.45, 4.86, and 3.17-fold of caffeic acid, 3,4-dihydroxybenzoic acid, p-coumaric acid, 4 hydroxybenzoic acid and ferulic acid, respectively. An associated increase in the total phenolic acid content and antioxidant activity in the <3 kDa fraction was obtained. In contrast with digestion, inhibition of advanced glycation end products formation and low-density lipoprotein oxidation was found in the <30 kDa fraction indicating the contribution of larger, possibly feruloylated polysaccharides, to activity. Cellular antioxidant activity in Caco-2 cells was >90% for all UD fractions, but with GDD was reduced. All fractions had low scavenging of nitric oxide in the lipopolysaccharide/murine cell model. Exposure of 3T3-L1 adipocytes to all the UD and GDD mageu fractions (at 1% and 10% concentrations) during differentiation resulted in at least a 35% reduction in lipid accumulation, which was not associated with a loss of cellular viability. In conclusion, mageu, UD, and subjected to GDD contains phenolic acids with beneficial bioactive properties that contribute to antioxidant activity and reduces lipid accumulation in adipocytes.
中文翻译：
商业样品体外消化的 mageu 的 3T3-L1 脂肪细胞中的抗氧化特性和脂质形成的抑制作用
Mageu 是一种发酵的、不含酒精的玉米衍生产品，是南部非洲独有的产品。本研究的目的是确定商业生产的 mageu 多酚提取物的健康益处，这些提取物与抗氧化特性和对分化的 3T3-L1 脂肪细胞中脂质积累的影响有关。对 mageu 1 号品牌（原始无味）的混合样品进行体外胃十二指肠消化 (GDD)。未分级未消化物 (UD) 和 GDD mageu 的反相高效液相色谱显示，通过消化，咖啡酸、3,4-二羟基苯甲酸、p-香豆酸、4-羟基苯甲酸和阿魏酸。获得了 <3 kDa 级分中总酚酸含量和抗氧化活性的相关增加。与消化相反，在 <30 kDa 部分发现了对晚期糖基化终产物形成和低密度脂蛋白氧化的抑制，表明较大的可能阿魏酰化多糖对活性的贡献。对于所有 UD 级分，Caco-2 细胞中的细胞抗氧化活性 >90%，但 GDD 降低。在脂多糖/鼠细胞模型中，所有级分都具有低的一氧化氮清除率。在分化期间将 3T3-L1 脂肪细胞暴露于所有 UD 和 GDD mageu 部分（浓度为 1% 和 10%）导致脂质积累至少减少 35%，这与细胞活力的丧失无关。总之，mageu、UD 和 GDD 含有酚酸，具有有益的生物活性特性，有助于抗氧化活性并减少脂肪细胞中的脂质积累。
更新日期：2021-10-06
中文翻译：
商业样品体外消化的 mageu 的 3T3-L1 脂肪细胞中的抗氧化特性和脂质形成的抑制作用
Mageu 是一种发酵的、不含酒精的玉米衍生产品，是南部非洲独有的产品。本研究的目的是确定商业生产的 mageu 多酚提取物的健康益处，这些提取物与抗氧化特性和对分化的 3T3-L1 脂肪细胞中脂质积累的影响有关。对 mageu 1 号品牌（原始无味）的混合样品进行体外胃十二指肠消化 (GDD)。未分级未消化物 (UD) 和 GDD mageu 的反相高效液相色谱显示，通过消化，咖啡酸、3,4-二羟基苯甲酸、p-香豆酸、4-羟基苯甲酸和阿魏酸。获得了 <3 kDa 级分中总酚酸含量和抗氧化活性的相关增加。与消化相反，在 <30 kDa 部分发现了对晚期糖基化终产物形成和低密度脂蛋白氧化的抑制，表明较大的可能阿魏酰化多糖对活性的贡献。对于所有 UD 级分，Caco-2 细胞中的细胞抗氧化活性 >90%，但 GDD 降低。在脂多糖/鼠细胞模型中，所有级分都具有低的一氧化氮清除率。在分化期间将 3T3-L1 脂肪细胞暴露于所有 UD 和 GDD mageu 部分（浓度为 1% 和 10%）导致脂质积累至少减少 35%，这与细胞活力的丧失无关。总之，mageu、UD 和 GDD 含有酚酸，具有有益的生物活性特性，有助于抗氧化活性并减少脂肪细胞中的脂质积累。
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