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A pH Self-Monitoring Heterogeneous Multicompartmental Proteinosome with Spatiotemporal Regulation of Insulin Transportation
Chinese Journal of Chemistry ( IF 5.5 ) Pub Date : 2021-09-14 , DOI: 10.1002/cjoc.202100519 Ping Wen 1 , Xueyi Wang 2 , Haixu Chen 1 , Dietmar Appelhans 3 , Xiaoman Liu 1 , Lei Wang 1 , Xin Huang 1
Chinese Journal of Chemistry ( IF 5.5 ) Pub Date : 2021-09-14 , DOI: 10.1002/cjoc.202100519 Ping Wen 1 , Xueyi Wang 2 , Haixu Chen 1 , Dietmar Appelhans 3 , Xiaoman Liu 1 , Lei Wang 1 , Xin Huang 1
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Inspired by the intricate eukaryotic cell structure, the spontaneously assembling multicompartments capable of spatiotemporally regulated biomacromolecules transportation is still a challenge. Here, a heterogeneous proteinosome-based multicompartment was designed and constructed by combining aqueous two-phase system and Pickering emulsion methods. By loading PEGylated insulin with boronic acid group into the multicompartment and glucose oxidase (GOx) into the innermost microgel domain, a short- or long-term transportation pathway of the loaded insulin was constructed by responding to the concentration of glucose. Moreover, the diminution of dynamic boronic ester bonding, the hindrance of polyethylene oxide micro-chamber, electrostatic interaction and the swelling behavior of hydrogel triggered by GOx/glucose reaction resulted in spatiotemporally controlled multi-pathway transportation mode with the releasing of insulin loaded inside the innermost microgel at high concentration of glucose (10 mg·mL–1). Specially, by incorporating rhodamine B and fluorescein labelled BSA into the multicompartment, the release procedure of the loaded insulin can be monitored by the fluorescence color-change. Overall, a multimode microcompartment is constructed which is then expected to provide a promising platform for further therapeutic protein, gene and drug delivery, as well as towards the design of complicated cell biomimetics.
中文翻译：
具有胰岛素运输时空调节的 pH 自我监测异质多室蛋白体
受错综复杂的真核细胞结构的启发，能够进行时空调控的生物大分子运输的自发组装多室仍然是一个挑战。在这里，通过结合水性两相系统和 Pickering 乳液方法，设计并构建了一种基于异质蛋白体的多室。通过将带有硼酸基团的聚乙二醇化胰岛素加载到多隔室中，将葡萄糖氧化酶 (GOx) 加载到最内部的微凝胶域中，通过响应葡萄糖浓度构建了加载胰岛素的短期或长期运输途径。此外，动态硼酸酯键的减少，聚环氧乙烷微室的阻碍，–1 )。特别地，通过将罗丹明 B 和荧光素标记的 BSA 加入多隔室，可以通过荧光颜色变化监测加载胰岛素的释放过程。总体而言，构建了一个多模式微室，然后有望为进一步的治疗性蛋白质、基因和药物递送以及复杂细胞仿生学的设计提供一个有前景的平台。
更新日期：2021-11-15
中文翻译：
具有胰岛素运输时空调节的 pH 自我监测异质多室蛋白体
受错综复杂的真核细胞结构的启发，能够进行时空调控的生物大分子运输的自发组装多室仍然是一个挑战。在这里，通过结合水性两相系统和 Pickering 乳液方法，设计并构建了一种基于异质蛋白体的多室。通过将带有硼酸基团的聚乙二醇化胰岛素加载到多隔室中，将葡萄糖氧化酶 (GOx) 加载到最内部的微凝胶域中，通过响应葡萄糖浓度构建了加载胰岛素的短期或长期运输途径。此外，动态硼酸酯键的减少，聚环氧乙烷微室的阻碍，–1 )。特别地，通过将罗丹明 B 和荧光素标记的 BSA 加入多隔室，可以通过荧光颜色变化监测加载胰岛素的释放过程。总体而言，构建了一个多模式微室，然后有望为进一步的治疗性蛋白质、基因和药物递送以及复杂细胞仿生学的设计提供一个有前景的平台。
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