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Ecofriendly/Rapid Synthesis of Silver Nanoparticles Using Extract of Waste Parts of Artichoke (Cynara scolymus L.) and Evaluation of their Cytotoxic and Antibacterial Activities
Journal of Nanomaterials Pub Date : 2021-09-14 , DOI: 10.1155/2021/2270472
Ayşe Baran 1 , Mehmet Fırat Baran 2 , Cumali Keskin 2 , Sevgi Irtegun Kandemir 3 , Mahbuba Valiyeva 4 , Sevil Mehraliyeva 4 , Rovshan Khalilov 5, 6, 7 , Aziz Eftekhari 7, 8
Affiliation  

Recycling wastes and providing their use in useful fields attract attention every day. In our study, with the extract prepared from the parts of the Cynara scolymus L. (artichoke) plant that is not suitable for human consumption, silver nanoparticles were easily synthesized in an ec-friendly, energy-free way. Characterization of the obtained nanoparticles was done with a UV-visible spectrophotometer (UV-Vis.), fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), and zeta potential analysis data. In these data, it was determined that AgNPs have a maximum absorbance at 458.8 nm wavelength, a crystal nanosize of 28.78 nm, and a spherical appearance. The zeta potential of (-) 16.9 mV indicates that silver nanoparticles exhibit a stable structure. Particles show antimicrobial effects on pathogenic species at concentrations of 0.03-0.25 μg/ml, and it was determined by using the minimum inhibition concentration (MIC) microdilution method. By examining their cytotoxic effects on U118, CaCo-2, and Skov-3 cancer cell lines and healthy HDF cell lines by the MTT method, concentrations of inhibitive effects on survival were determined.

中文翻译:

使用朝鲜蓟 (Cynara scolymus L.) 废料提取物的生态友好/快速合成银纳米颗粒及其细胞毒性和抗菌活性评价

回收废物并将其用于有用的领域每天都受到关注。在我们的研究中,使用从Cynara scolymus部分制备的提取物L.(朝鲜蓟)植物,不适合人类食用,银纳米颗粒很容易以环保、无能源的方式合成。使用紫外-可见分光光度计 (UV-Vis.)、傅里叶变换红外光谱 (FTIR)、X 射线衍射仪 (XRD)、扫描电子显微镜 (SEM)、透射电子显微镜 (TEM) 对所获得的纳米颗粒进行表征和 zeta 电位分析数据。在这些数据中,确定 AgNPs 在 458.8 nm 波长处具有最大吸光度,晶体纳米尺寸为 28.78 nm,外观呈球形。(-) 16.9 mV的zeta电位表明银纳米颗粒表现出稳定的结构。颗粒在 0.03-0.25 μ浓度下对病原体物种显示出抗菌作用 g/ml,采用最小抑制浓度(MIC)微量稀释法测定。通过 MTT 方法检查它们对 U118、CaCo-2 和 Skov-3 癌细胞系和健康 HDF 细胞系的细胞毒性作用,确定了对存活的抑制作用浓度。
更新日期:2021-09-14
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