The formation of embryonic muscle fibers determines the amount of postnatal muscles and is regulated by a variety of signaling pathways and transcription factors. Previously, by using chromatin immunoprecipitation-sequencing and RNA-Seq techniques, we identified a large number of genes that are regulated by H3K27me3 in porcine embryonic skeletal muscles. Among these genes, we found that ITGB6 is regulated by H3K27me3. However, its function in muscle development is unknown. In this study, we first verified that ITGB6 was differentially regulated by H3K27me3 and that its expression levels were upregulated in porcine skeletal muscles at embryonic Days 33, 65, and 90. Then, we performed gain- or loss-of-function studies on porcine skeletal muscle satellite cells to study the role of ITGB6 in porcine skeletal muscle development. The proliferation of porcine skeletal muscle satellite cells was studied through real-time polymerase chain reaction, Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine staining, Western blot, and flow cytometry analyses. We found that the ITGB6 gene was regulated by H3K27me3 during muscle development and had an inhibitory effect on the proliferation of porcine skeletal muscle satellite cells.

中文翻译：

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ITGB6抑制猪骨骼肌卫星细胞增殖

胚胎肌纤维的形成决定了出生后肌肉的数量，并受多种信号通路和转录因子的调节。此前，通过使用染色质免疫沉淀测序和 RNA-Seq 技术，我们在猪胚胎骨骼肌中鉴定出大量受 H3K27me3 调控的基因。在这些基因中，我们发现ITGB6受 H3K27me3 调控。然而，它在肌肉发育中的作用是未知的。在这项研究中，我们首先验证了ITGB6受 H3K27me3 的差异调节，并且其表达水平在胚胎第 33、65 和 90 天在猪骨骼肌中上调。然后，我们对猪进行了功能增益或功能丧失研究研究骨骼肌卫星细胞的作用ITGB6在猪骨骼肌发育中的作用。通过实时聚合酶链反应、细胞计数 Kit-8、5-ethynyl-2′-脱氧尿苷染色、蛋白质印迹和流式细胞术分析研究了猪骨骼肌卫星细胞的增殖。我们发现ITGB6基因在肌肉发育过程中受H3K27me3调控，对猪骨骼肌卫星细胞的增殖有抑制作用。