Extract and fraction of Musa paradisiaca flower have osteogenic effect and prevent ovariectomy induced osteopenia,Phytomedicine

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Extract and fraction of Musa paradisiaca flower have osteogenic effect and prevent ovariectomy induced osteopenia
Phytomedicine ( IF 6.7 ) Pub Date : 2021-09-14 , DOI: 10.1016/j.phymed.2021.153750
Reena Rai ₁ , Sudhir Kumar ₂ , Krishna Bhan Singh ₃ , Sonu Khanka ₃ , Yatendra Singh ₄ , K R Arya ₅ , Sanjeev Kanojiya ₄ , Rakesh Maurya ₂ , Divya Singh ₃

Affiliation

Background

Osteoporosis is an asymptomatic bone disorder leading to altered bone microarchitecture, mineralization and strength. Musa paradisiaca has been reported to have antioxidant and anti-inflammatory effects in various diseases. Its impact on postmenopausal osteoporosis has not been investigated yet.

Purpose

The intention of the current study was to evaluate the bone regeneration and osteoprotective potential of extract and fraction of M. paradisiaca flower in ovariectomized (Ovx) Sprague Dawley (SD) rats, a model of post-menopausal bone loss. The study also aims to identify osteogenic compounds from active fraction.

Methods

Ethanolic extract (MFE) and butanolic fraction (MFE-Bu) from flower of M. paradisiaca were prepared and their efficacy was tested in rat femur osteotomy model at different doses. Effective dose from both extract (250 mg/kg) and fraction (50 mg/kg) were taken for study in osteopenic bone loss model. PTH was taken as reference standard (20 µg/kg/twice a week). Bones were harvested at autopsy for dynamic and static histomorphometry. Serum was collected for ELISA. Pure compounds were isolated from butanolic fraction (MFE-Bu), and were assessed for their osteogenic effect.

Results

MFE and MFE-Bu were observed for their potential in bone healing and prevention of bone loss. Both MFE and MFE-Bu promoted new bone regeneration at injury site as assessed by microCT and calcein dye labeling studies. These also led to restoration of bone microarchitecture deteriorated as a result of osteopenia and improved bone biomechanical properties. Extract as well as the fraction exhibited dual bone anabolic and anti-resorptive properties where they elevated serum procollagen type I N-terminal propeptide (P1NP), a bone formation marker and suppressed serum C-telopeptide of type I collagen (CTX-1), a bone resorption marker. As many as four osteogenic compounds were isolated from MFE-Bu. Oleracein-E was found to be the most potent osteogenic agent based on osteoblast differentiation, mineralization assays, qPCR and protein expression studies.

Conclusion

Our studies demonstrates that ethanolic extract from the flower of M. paradisiaca and its butanolic fraction exhibit dual osteogenic and anti-resorptive potential, and have an advantage over PTH which though promotes bone formation but is also bone catabolic in nature.

中文翻译：

Musa paradisiaca花的提取物和馏分具有成骨作用并防止卵巢切除引起的骨质减少

背景

骨质疏松症是一种无症状的骨骼疾病，导致骨骼微结构、矿化和强度发生改变。据报道，Musa paradisiaca在各种疾病中具有抗氧化和抗炎作用。尚未研究其对绝经后骨质疏松症的影响。

目的

本研究的目的是评估M. paradisiaca花提取物和组分在切除卵巢 (Ovx) 的 Sprague Dawley (SD) 大鼠（一种绝经后骨质流失模型）中的骨再生和骨保护潜力。该研究还旨在从活性成分中鉴定成骨化合物。

方法

从M. paradisiaca花中制备乙醇提取物 (MFE) 和丁醇组分 (MFE-Bu)，并在不同剂量的大鼠股骨截骨模型中测试其功效。提取物 (250 mg/kg) 和馏分 (50 mg/kg) 的有效剂量用于骨质减少性骨质流失模型的研究。PTH 作为参考标准（20 µg/kg/每周两次）。在尸检时采集骨骼用于动态和静态组织形态测量。收集血清用于ELISA。从丁醇级分 (MFE-Bu) 中分离出纯化合物，并评估其成骨作用。

结果

观察到 MFE 和 MFE-Bu 在骨愈合和预防骨丢失方面的潜力。通过显微CT和钙黄绿素染料标记研究评估，MFE和MFE-Bu都促进了损伤部位的新骨再生。这些也导致由于骨质减少和改善的骨生物力学特性而恶化的骨微结构的恢复。提取物以及该部分表现出双重骨合成代谢和抗吸收特性，其中它们提高了血清 I 型前胶原 N 端前肽 (P1NP)，一种骨形成标志物，并抑制了 I 型胶原的血清 C 端肽 (CTX-1)，骨吸收标志物。从 MFE-Bu 中分离出多达四种成骨化合物。根据成骨细胞分化、矿化分析、qPCR 和蛋白质表达研究，发现 Oleracein-E 是最有效的成骨剂。