Background: Previous studies have suggested that consuming fruit and vegetable can lower the risk of several cancers, including breast, colorectal, and lung cancers.
Aims: The present study aims to investigate the in vitro anticancer effects of Shahani and Asgari grape seed extract (GSE) grown in Malayer City of Iran on HL-60 cancer. However, to the best of the author's knowledge, it is the first time in this study that the antiproliferative effect of Shahani and Asgari GSE is compared.
Materials and Methods: Shahani and Asgari GSE Was extraction white method of Liquid/liquid extraction with ethyl acetate. Then assessing cytotoxic activities of Shahani and Asgari GSE on the HL-60 cells was tested using MTT assay.
Results: The results show that compared with the control group, seed extract of both Shahani and Asgari at the various concentrations (25, 50, 100, and 200 μg/ml) had a significantly inhibitory effect on HL-60 cell proliferation that was dose dependent. However, Shahani GSE at different concentrations (50, 100, and 200 μg/ml) indicated a significantly higher inhibitory effect compared to Asgari GSE. In addition, GSE can induce cell cycle arrest at G0/G1 cells. Furthermore, GSE of Asgari and Shahani remarkably increased the induction of HL-60 cell apoptosis depending on its dose. However, at the concentration of 200 μg/ml, GSE induced cell necrosis rather than apoptosis.
Conclusion: Seed extract of both Shahani and Asgari at the various concentrations had a significantly inhibitory effect on HL-60 cell proliferation that was dose dependent.


中文翻译：

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马来沙哈尼和阿斯加里葡萄籽提取物诱导人白血病细胞凋亡的相对作用


背景：以前的研究表明，食用水果和蔬菜可以降低患多种癌症的风险，包括乳腺癌、结直肠癌和肺癌。
目的：本研究旨在调查伊朗马来市种植的 Shahani 和 Asgari 葡萄籽提取物 (GSE) 对 HL-60 癌症的体外抗癌作用。然而，据作者所知，本研究首次比较 Shahani 和 Asgari GSE 的抗增殖作用。
材料与方法： Shahani 和 Asgari GSE 是用乙酸乙酯液/液萃取的白色萃取法。然后使用 MTT 法测试评估 Shahani 和 Asgari GSE 对 HL-60 细胞的细胞毒活性。
结果：结果表明，与对照组相比，不同浓度（25、50、100和200 μg/ml）的沙哈尼和阿斯加里种子提取物对HL-60细胞增殖具有明显的抑制作用，且呈剂量依赖性。然而，与 Asgari GSE 相比，不同浓度（50、100 和 200 μg/ml）的 Shahani GSE 显示出显着更高的抑制作用。此外，GSE 可以诱导 G0/G1 细胞的细胞周期停滞。此外，Asgari 和 Shahani 的 GSE 显着增加了 HL-60 细胞凋亡的诱导，这取决于其剂量。然而，在 200 μg/ml 的浓度下，GSE 诱导细胞坏死而不是细胞凋亡。
结论：不同浓度的 Shahani 和 Asgari 种子提取物对 HL-60 细胞增殖具有显着的抑制作用，且呈剂量依赖性。