Background and Purpose: Increased researches focus into pathophysiological mechanisms of spinal cord injury (SCI), particularly toward the relationship between relevant biomarkers and the degree of SCI and prognosis. Circular ribonucleic acids (circRNAs) possess microRNA (miRNA) binding sites that can play the role of miRNA sponges and thus participate in the expression of parental gene modification. This study focused on rat SCI models and explore the relationship between circRNAs and SCI at a genomic level. Methods: We first established a rat SCI model and extracted the target spinal cord tissue according to 4 time points. Then investigated the alterations in the circRNA expression by high-throughput whole transcriptome sequencing, analyzed data by gene ontology and the Kyoto Encyclopedia of Genes and Genomes, and constructed the circRNA-miRNA network. Results: A total of 178 circRNAs were dysregulated (89 upregulated/89 downregulated). Differential circRNAs were found to be mainly involved in the composition of specific organelles in the cytoplasm and are mainly involved in the energy transfer process associated with electron transfer (and similar activities). In all the signaling pathways identified in this study, the MAPK, Wnt, and mTOR signaling pathways are intimately associated with the pathophysiological process of rats post-SCI. In this study, 10 circRNAs with obvious dysregulation were selected for prediction, 26 miRNAs with additional interactions were obtained, and a network diagram of circRNAs-miRNAs was constructed. In this manner, one can understand in further detail the pathogenesis of SCI and to provide new strategies for the prevention, diagnosis, and treatment of SCI-related injuries at the genetic level.
Neuroimmunomodulation

中文翻译：

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大鼠脊髓损伤后脊髓组织中环状核糖核酸表达的改变

背景与目的：越来越多的研究集中在脊髓损伤 (SCI) 的病理生理机制，特别是相关生物标志物与 SCI 程度和预后之间的关系。环状核糖核酸（circRNAs）具有微小RNA（miRNA）结合位点，可以发挥miRNA海绵的作用，从而参与亲本基因修饰的表达。本研究侧重于大鼠 SCI 模型，并在基因组水平上探索 circRNA 与 SCI 之间的关系。方法：我们首先建立大鼠SCI模型，并根据4个时间点提取目标脊髓组织。然后通过高通量全转录组测序研究circRNA表达的变化，通过基因本体和京都基因与基因组百科全书分析数据，构建circRNA-miRNA网络。结果：共有 178 个 circRNA 失调（89 个上调/89 个下调）。发现差异环状RNA主要参与细胞质中特定细胞器的组成，主要参与与电子转移（和类似活动）相关的能量转移过程。在本研究确定的所有信号通路中，MAPK、Wnt 和 mTOR 信号通路与大鼠 SCI 后的病理生理过程密切相关。本研究选取10个明显失调的circRNAs进行预测，得到26个额外相互作用的miRNAs，构建了circRNAs-miRNAs的网络图。通过这种方式，人们可以更详细地了解 SCI 的发病机制，并为预防、诊断、
神经免疫调节