In this study, cellulose nanocrystals (CNC) as functional cross-linker and Pickering emulsifier was used to stabilize Lysozyme (Lys) encapsulated in quaternary ammonium chitosan nanoparticles (QC NPs) via ionic gelation method. Physicochemical, structural, and antibacterial properties of the CNC stabilized Lys loaded QC NPs were also evaluated. Particle size, particle size distribution, Zeta potential (ZP), and spectroscopic analyses showed the successful encapsulation of Lys. Antibacterial activity of NPs against Staphylococcus aureus and Vibrio parahaemolyticus was investigated on the basis of inhibition zone (IZ), minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). MIC and MBC of CNC stabilized Lys loaded HQC NPs against S. aureus were 0.094 and 0.188 while corresponding values for CNC stabilized Lys loaded LQC NPs V. parahaemolyticus were 0.156 and 0.312 mg/mL, respectively. Therefore, CNC stabilized Lys loaded QC NPs have potential implications in the food industry for food preservation and packaging.

在这项研究中，纤维素纳米晶体 (CNC) 作为功能性交联剂和皮克林乳化剂用于通过离子凝胶法稳定包裹在季铵壳聚糖纳米颗粒 (QC NPs) 中的溶菌酶 (Lys)。还评估了 CNC 稳定的 Lys 负载 QC NP 的物理化学、结构和抗菌性能。粒度、粒度分布、Zeta 电位 (ZP) 和光谱分析表明 Lys 成功包封。根据抑菌圈 (IZ)、最小抑菌浓度 (MIC) 和最小细菌浓度 (MBC) 研究了 NPs 对金黄色葡萄球菌和副溶血性弧菌的抗菌活性。CNC 的 MIC 和 MBC 稳定负载 Lys 的 HQC NPs 对抗金黄色葡萄球菌分别为 0.094 和 0.188，而 CNC 稳定的 Lys 负载 LQC NPs副溶血性弧菌的相应值分别为 0.156 和 0.312 mg/mL。因此，CNC 稳定的 Lys 负载 QC NPs 在食品行业的食品保存和包装方面具有潜在意义。