To better comprehend the physiology of cephalopods, we used a minimal invasive technique of skin mucus swabs to measure immunoreactive corticosteroids in three cephalopod species commonly kept in captivity and promoted as new model organisms: Euprymna berryi, Sepia bandensis, and Octopus chierchiae. We compared results between sexes and age classes and then evaluated their stress responses during acclimation to a new habitat. To better understand glucocorticoid production, we conducted an adrenocorticotropic hormone, using Cosyntropin (an adrenocorticotropin (ACTH) analogue) challenge with a saline control and swabbed their mantles at 15-minute intervals for 2 h. Results showed cortisol was higher for younger individuals. Additionally, cortisol and corticosterone concentrations decreased by 2-fold after 2 to 4 days of acclimation to a new habitat. We were able to successfully measure 2-fold increase in immunoreactive corticosteroids which reacted with cortisol and corticosterone assays for all the species following ACTH injection, although not all individuals responded similarly. With further investigation, this technique can increase our understanding and management of cephalopods in captivity.

为了更好地理解头足类动物的生理学，我们使用皮肤粘液拭子的微创技术来测量三种通常被圈养并作为新模式生物推广的头足类动物的免疫反应性皮质类固醇：Euprymna berryi、Sepia bandensis和Octopus chierchiae. 我们比较了性别和年龄组之间的结果，然后评估了他们在适应新栖息地期间的压力反应。为了更好地了解糖皮质激素的产生，我们进行了促肾上腺皮质激素，使用 Cosyntropin（一种促肾上腺皮质激素 (ACTH) 类似物）和盐水对照，并以 15 分钟的间隔擦拭它们的外套膜 2 小时。结果显示，年轻人的皮质醇含量更高。此外，在适应新栖息地 2 至 4 天后，皮质醇和皮质酮浓度下降了 2 倍。在 ACTH 注射后，我们能够成功地测量到与皮质醇和皮质酮测定反应的免疫反应性皮质类固醇增加了 2 倍，尽管并非所有个体的反应都相似。随着进一步调查，