当前位置: X-MOL 学术ACS Appl. Polym. Mater. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Conjugated Bio-Polymer Anchored Surfaces to Mitigate Stain and Bacterial Colonization for Oral Hygiene Application
ACS Applied Polymer Materials ( IF 4.4 ) Pub Date : 2021-09-14 , DOI: 10.1021/acsapm.0c01323
Shabnam Pathan 1 , Paresh Kumar Samantaray 2 , Sagar Nilawar 1 , Sumana Roychowdhury 3 , Somnath Das 3 , Satyajit Samadder 3 , Amitava Pramanik 3 , Kaushik Chatterjee 1 , Suryasarathi Bose 1
Affiliation  

Due to health and environmental hazards, there is an increased desire to rely on sustainable resources that are biocompatible, biodegradable, and safe for human administration. Herein, the present study explores bioconjugate efficacy as a potential anti-stain and green antibacterial additive in toothpaste formulation using GRAS (generally regarded as safe) and Food and Drug Administration (FDA)-approved ingredients. In this study, a series of conjugated polymers were synthesized using sodium alginate and alendronic acid and are labeled as SA-ALN amide and SA-ALN ester. Spectroscopic techniques thoroughly characterized the synthesized conjugated polymers. These synthesized polymers were adsorbed onto hydroxyapatite (HAP, a tooth model) to evaluate its practical application as anti-stain and reduce bacterial attachment. The adsorption of the conjugate polymers on the HAP surface was confirmed by Raman spectra and Fourier transform infrared spectroscopy (FTIR). The anti-stain test revealed significant whitening benefits for coated hydroxyapatite (HAP) tiles over uncoated tiles. The antibacterial activity of conjugated polymers toward E. coli (Gram-negative) and S. mutans (Gram-positive, bacteria responsible for tooth decay) strain showed 2.5 log reduction and 1.5 log reduction, respectively. Among the conjugated polymers, the SA-ALN-ester showed slightly higher antibacterial activity against E. coli as well as S. mutans strain relative to the SA-ALN amide product due to the presence of a free amine group in the former. Further, we carried out the in vitro cytotoxicity test of the conjugated polymers using gingival fibroblasts and pre-osteoblasts and the study demonstrated that the synthesized conjugates are cytocompatible in nature. Thus, this study revealed that the proposed work might provide insights into sustainable oral hygiene.

中文翻译:

共轭生物聚合物锚定表面可减轻口腔卫生应用中的污渍和细菌定植

由于健康和环境危害,人们越来越希望依赖具有生物相容性、可生物降解和对人类给药安全的可持续资源。在此,本研究使用 GRAS(通常被认为是安全的)和食品和药物管理局 (FDA) 批准的成分,探讨了生物共轭物作为潜在的抗污剂和绿色抗菌添加剂在牙膏配方中的功效。在这项研究中,使用海藻酸钠和阿仑膦酸合成了一系列共轭聚合物,并标记为 SA-ALN 酰胺和 SA-ALN 酯。光谱技术彻底表征了合成的共轭聚合物。这些合成的聚合物被吸附到羟基磷灰石(HAP,一种牙齿模型)上,以评估其作为抗污剂和减少细菌附着的实际应用。通过拉曼光谱和傅里叶变换红外光谱 (FTIR) 证实了共轭聚合物在 HAP 表面上的吸附。防污测试表明,涂层羟基磷灰石 (HAP) 瓷砖比未涂层瓷砖具有显着的增白效果。共轭聚合物的抗菌活性大肠杆菌(革兰氏阴性)和变形链球菌(革兰氏阳性,导致蛀牙的细菌)菌株分别显示出 2.5 log 减少和 1.5 log 减少。在共轭聚合物中,SA-ALN-酯对大肠杆菌变形链球菌菌株的抗菌活性略高于SA-ALN酰胺产物,因为前者中存在游离胺基团。此外,我们使用牙龈成纤维细胞和前成骨细胞对共轭聚合物进行了体外细胞毒性测试,研究表明合成的共轭物本质上是细胞相容的。因此,这项研究表明,拟议的工作可能会提供对可持续口腔卫生的见解。
更新日期:2021-10-08
down
wechat
bug