Choroid plexus (CP) is the principal source of cerebrospinal fluid. CP can produce and release a wide range of materials including growth factors, neurotrophic factors, etc. all of which play an important role in the maintenance and proper functioning of the brain. Methamphetamine (METH) is a CNS neurostimulant that causes brain dysfunction. Herein, we investigated the potential effects of METH exposure on CP structure and function. Stereological analysis revealed a significant alteration in CP volume, epithelial cells and capillary number upon METH treatment. Electron microscopy exhibited changes in ultrastructure. Moreover, the upregulation of neurotrophic factors such as BDNF and VEGF as well as autophagy and apoptosis gene following METH administration were observed. We also identified several signaling cascades related to autophagy. In conclusion, gene expression changes coupled with structural alterations of the CP in response to METH suggested METH‐induced autophagy in CP.

脉络丛（CP）是脑脊液的主要来源。CP可以产生和释放多种物质，包括生长因子、神经营养因子等，所有这些物质对大脑的维持和正常运作都起着重要作用。甲基苯丙胺 (METH) 是一种 CNS 神经兴奋剂，可导致脑功能障碍。在此，我们研究了 METH 暴露对 CP 结构和功能的潜在影响。体视学分析显示 METH 治疗后 CP 体积、上皮细胞和毛细血管数量发生显着变化。电子显微镜显示超微结构的变化。此外，观察到 METH 给药后神经营养因子如 BDNF 和 VEGF 以及自噬和凋亡基因的上调。我们还确定了几个与自噬相关的信号级联。