Three-dimensional (3D) cell culture enhanced cells-cells communication, promoting developmental processes and influencing cell performance. In this study, 3D alginate-gelatin core–shell capsules (AGC) were fabricated by using a microfluidic device. Human osteoblast-like MG-63 cells were encapsulated within the 3D model. The morphology and physicochemical properties of the AGC were characterized by using scanning electron microscopy (SEM), Flourier Transform Infrared Spectrum (FTIR), Gravimetric thermal analyzer (TGA). The mRNA levels of osteogenetic gene markers were measured by quantitative real-time RT-PCR. The result of a 9.6% weight loss after a 7-day soaking indicates that the AGC exhibited excellent stability. The encapsulated MG-63 cells formed clusters alongside the core of AGC and obtained 200% cell viability after 21-day long-term cultivation. Compared to traditional 2D cell culture, the 3D cultured MG-63 cells performed higher alkaline phosphatase (ALP), a 9.3-fold increase of calcium mineralization activity, and enhanced osteogenic gene expression of BGLAP, COL1A1, and Runx2. These observations suggested that AGC obtains the potential as an ideal 3D microenvironmental cell culture system for biomedical application.

三维 (3D) 细胞培养增强了细胞间通讯，促进了发育过程并影响了细胞性能。在这项研究中，3D 藻酸盐-明胶核壳胶囊 (AGC) 是通过使用微流体装置制造的。人类成骨细胞样 MG-63 细胞被封装在 3D 模型中。通过使用扫描电子显微镜 (SEM)、弗洛里叶变换红外光谱 (FTIR)、重量热分析仪 (TGA) 对 AGC 的形貌和理化性质进行了表征。通过定量实时RT-PCR测量成骨基因标记物的mRNA水平。浸泡 7 天后重量损失 9.6% 的结果表明 AGC 表现出优异的稳定性。封装后的MG-63细胞与AGC核心形成簇状，经过21天的长期培养获得200%的细胞活力。与传统的 2D 细胞培养相比，3D 培养的 MG-63 细胞表现出更高的碱性磷酸酶 (ALP)，钙矿化活性增加 9.3 倍，并增强BGLAP、COL1A1和Runx2 的成骨基因表达。这些观察结果表明，AGC 具有作为用于生物医学应用的理想 3D 微环境细胞培养系统的潜力。