Abstract

Despite its similarity to protein biosynthesis in bacteria, translation in the mitochondria of modern eukaryotes has several unique features, such as the necessity for coordination of translation of mitochondrial mRNAs encoding proteins of the electron transport chain complexes with translation of other protein components of these complexes in the cytosol. In the mitochondria of baker’s yeast Saccharomyces cerevisiae, this coordination is carried out by a system of translational activators that predominantly interact with the 5′-untranslated regions of mitochondrial mRNAs. No such system has been found in human mitochondria, except a single identified translational activator, TACO1. Here, we studied the role of the ZMYND17 gene, an ortholog of the yeast gene for the translational activator Mss51p, on the mitochondrial translation in human cells. Deletion of the ZMYND17 gene did not affect translation in the mitochondria, but led to the decrease in the cytochrome c oxidase activity and increase in the amount of free F₁ subunit of ATP synthase. We also investigated the evolutionary history of Mss51p and ZMYND17 and suggested a possible mechanism for the divergence of functions of these orthologous proteins.

中文翻译：

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酵母翻译激活剂 Mss51p 和人类 ZMYND17 – 两种具有共同起源但功能不同的蛋白质

 抽象的

尽管与细菌中的蛋白质生物合成相似，但现代真核生物线粒体中的翻译具有几个独特的特征，例如编码电子传递链复合物蛋白质的线粒体 mRNA 的翻译与这些复合物的其他蛋白质成分的翻译之间的协调必要性。细胞质。在面包酵母酿酒酵母的线粒体中，这种协调是由翻译激活剂系统进行的，该系统主要与线粒体 mRNA 的 5'-非翻译区相互作用。除了一个已鉴定的翻译激活因子 TACO1 之外，在人类线粒体中尚未发现这样的系统。在这里，我们研究了ZMYND17基因（翻译激活子 Mss51p 的酵母基因的直系同源物）在人类细胞线粒体翻译中的作用。 ZMYND17基因的缺失并不影响线粒体中的翻译，但导致细胞色素c氧化酶活性降低和ATP合酶游离F ₁亚基数量增加。我们还研究了 Mss51p 和 ZMYND17 的进化历史，并提出了这些直系同源蛋白功能差异的可能机制。