Stem Cell Reviews and Reports ( IF 4.5 ) Pub Date : 2021-09-13 , DOI: 10.1007/s12015-021-10249-0 Christian Olszewski 1 , Jessika Maassen 1 , Rebecca Guenther 1 , Claudia Skazik-Voogt 1 , Angela Gutermuth 1
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Corneal endothelial insufficiency is one of the leading causes of blindness. The main contemporary treatment for corneal blindness is endothelial keratoplasty, which, however, is unsatisfactory as a medical therapy due to the lack of donor corneas and graft rejection. Therefore, autologous stem cell-based corneal endothelial tissue substitutes may be a promising alternative to conventional grafts in the future. To address the age of most patients suffering from corneal endothelial deficiencies, we investigated the presence and potential of hair-derived stem cells from older tissue donors. Our studies revealed the presence of pluripotency- and neural crest-associated markers in tissue sections from blepharoplasty patients aged 50 to 80 years. In vitro outgrowths from eyelid hair follicles on collagen-coated tissue culture plates revealed a weak decrease in stem-cell potency. In contrast, cells within the spheres that spontaneously formed from the adherent cell layer retained full stem-cell potency and could be differentiated into cells of the ecto- meso and endodermal lineages. Although these highly potent hair follicle derived stem cells (HFSC) were only very slightly expandable, they were able to recognize the biomimicry of the Descemet’s-like topography and differentiate into corneal endothelial-like cells. In conclusion, HFSCs derived from epidermal skin of eyelid biopsies are a promising cell source to provide autologous corneal endothelial replacement for any age group of patients.
Graphical Abstract
中文翻译：
来自老化眼睑皮肤的毛囊干细胞向角膜内皮样细胞的机械传导分化
角膜内皮功能不全是导致失明的主要原因之一。角膜失明的主要当代治疗方法是内皮角膜移植术，然而，由于缺乏供体角膜和移植物排斥，其作为一种医学疗法并不令人满意。因此，基于自体干细胞的角膜内皮组织替代物可能是未来传统移植物的有希望的替代品。为了解决大多数角膜内皮缺陷患者的年龄问题，我们调查了来自老年组织供体的毛发干细胞的存在和潜力。我们的研究揭示了 50 至 80 岁眼睑成形术患者的组织切片中存在多能性和神经嵴相关标志物。胶原蛋白包被的组织培养板上眼睑毛囊的体外生长表明干细胞效力的弱下降。相比之下，从贴壁细胞层自发形成的球体内的细胞保留了完整的干细胞潜能，并且可以分化成外胚层和内胚层谱系的细胞。尽管这些高效的毛囊衍生干细胞 (HFSC) 的可扩展性非常小，但它们能够识别后弹力样地形的仿生学并分化成角膜内皮样细胞。总之，来自眼睑活检表皮的 HFSC 是一种很有前途的细胞来源，可为任何年龄组的患者提供自体角膜内皮替代物。由贴壁细胞层自发形成的球体内的细胞保留了完整的干细胞潜能，并且可以分化成外胚层和内胚层谱系的细胞。尽管这些高效的毛囊衍生干细胞 (HFSC) 的可扩展性非常小，但它们能够识别后弹力样地形的仿生学并分化成角膜内皮样细胞。总之，来自眼睑活检表皮的 HFSC 是一种很有前途的细胞来源，可为任何年龄组的患者提供自体角膜内皮替代物。由贴壁细胞层自发形成的球体内的细胞保留了完整的干细胞潜能，并且可以分化成外胚层和内胚层谱系的细胞。尽管这些高效的毛囊衍生干细胞 (HFSC) 的可扩展性非常小，但它们能够识别后弹力样地形的仿生学并分化成角膜内皮样细胞。总之，来自眼睑活检表皮的 HFSC 是一种很有前途的细胞来源，可为任何年龄组的患者提供自体角膜内皮替代物。他们能够识别后弹力样地形的仿生学并分化成角膜内皮样细胞。总之，来自眼睑活检表皮的 HFSC 是一种很有前途的细胞来源，可为任何年龄组的患者提供自体角膜内皮替代物。他们能够识别后弹力样地形的仿生学并分化成角膜内皮样细胞。总之，来自眼睑活检表皮的 HFSC 是一种很有前途的细胞来源，可为任何年龄组的患者提供自体角膜内皮替代物。
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