Xylanases with high specific activity has been focused with great interest as a useful enzyme in biomass utilization. The production of recombinant GH11 xylanase (MYCTH_56237) from Myceliophthora thermophila has been improved through N-terminal signal peptide engineering in P. pastoris. The production of newly recombinant xylanase (termed Mtxyn11C) was improved from 442.53 to 490.7 U/mL, through a replacement of α-factor signal peptide with the native xylanase signal peptide segment (MVSVKAVLLLGAAGTTLA) in P. pastoris. Scaling up of Mtxyn11C production in a 7.5 L fermentor was improved to the maximal production rate of 2503 U/mL. In this study, the degradation efficiency of Mtxyn11C was further examined. Analysis of the hydrolytic mode of action towards the birchwood xylan (BWX) revealed that Mtxyn11C was clearly more effective than commercial xylanase and degrades xylan into xylooligosaccharides (xylobiose, xylotriose, xylotetraose). More importantly, Mtxyn11C in combination with a single multifunctional xylanolytic enzyme, improved the hydrolysis of BWX into single xylose by 40%. Altogether, this study provided strategies for improved production of xylanase together with rapid conversion of xylose from BWX, which provides sustainable, cost-effective and environmental friendly approaches to produce xylose/XOSs for biomass energy or biofuels production.

中文翻译：

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毕赤酵母中木聚糖酶产量的提高及其在木聚糖生产中的应用。

具有高比活性的木聚糖酶作为生物质利用中的有用酶引起了极大的兴趣。嗜热毁丝霉中重组 GH11 木聚糖酶 (MYCTH_56237) 的生产已通过 P. pastoris 中的 N 端信号肽工程得到改进。通过用巴斯德毕赤酵母中的天然木聚糖酶信号肽段 (MVSVKAVLLLGAAGTTLA) 替换 α 因子信号肽，新重组木聚糖酶（称为 Mtxyn11C）的产量从 442.53 U/mL 提高到 490.7 U/mL。Mtxyn11C 在 7.5 L 发酵罐中的生产规模扩大到 2503 U/mL 的最大生产速率。在本研究中，进一步检查了 Mtxyn11C 的降解效率。对桦木木聚糖 (BWX) 的水解作用模式分析表明，Mtxyn11C 显然比商业木聚糖酶更有效，并将木聚糖降解为低聚木糖（木二糖、木三糖、木四糖）。更重要的是，Mtxyn11C 与单一的多功能木聚糖分解酶结合，将 BWX 水解为单一木糖的速度提高了 40%。总而言之，这项研究提供了提高木聚糖酶产量以及从 BWX 中快速转化木糖的策略，这为生产用于生物质能源或生物燃料生产的木糖/XOS 提供了可持续、具有成本效益和环境友好的方法。将 BWX 水解成单一木糖的速度提高了 40%。总而言之，这项研究提供了提高木聚糖酶产量以及从 BWX 中快速转化木糖的策略，这为生产用于生物质能源或生物燃料生产的木糖/XOS 提供了可持续、具有成本效益和环境友好的方法。将 BWX 水解成单一木糖的速度提高了 40%。总而言之，这项研究提供了提高木聚糖酶产量以及从 BWX 中快速转化木糖的策略，这为生产用于生物质能源或生物燃料生产的木糖/XOS 提供了可持续、具有成本效益和环境友好的方法。